| 杭菊花茎尖组织培养初探 |
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| 引用本文： | 王康才,茅毓英,张雪琼.杭菊花茎尖组织培养初探[J].中药材,2000,23(3):125-127. |
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| 作者姓名： | 王康才 茅毓英 张雪琼 |
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| 作者单位： | 南京农业大学,南京210095 |
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| 摘 要： | 为解决杭菊的品种退化问题，以大白菊，小白菊，黄菊三个品种类型的茎尖为外植体，接种在不同ＰＨ的ＭＳ培养其及不同激素配比的培养基上。结果表明，ＰＨ＝５．８－６．４是外植体诱愈率在８０％以上；外体植接种在不同激素与比的ＭＳ培养基上，ＮＡＡ为０．２ｍｇ／Ｌ时ＫＴ的诱愈率明显高于６－ＢＡ。诱导生根的适宜培养基是１／２ＭＳ＋ＮＡＡ０．２ｍｇ／Ｌ。
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| 关 键 词： | 杭菊花 茎尖 组织培养 |
| 修稿时间： | 1999-07-05 |
A Preliminary Study on Tissue Culture of Stem Apex of Chrysanthemum morifolium |
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| Wang Kangcai,Mao Yuying,Zhang Xueqiong.A Preliminary Study on Tissue Culture of Stem Apex of Chrysanthemum morifolium[J].Jorunal of Chinese Medicinal Materials,2000,23(3):125-127. |
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| Authors: | Wang Kangcai Mao Yuying Zhang Xueqiong |
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| Institution: | Wang Kangcai
,Mao Yuying
,Zhang Xueqiong
(Depamnent of Horticulture, Nanjing Agricultural University, Nanjing 210095) |
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| Abstract: | To solve the breed degeneration of Chrysanthemum the stem apex of Chrysanthemum morifolium used as explants were inoculated on MS medium with different pH and supplemented with different ratio of plant hormone. The results showed that when pH was between 5.8 and 6.4, the rate of callus were all over 80%. While explants inoculated on MS medium supplemented with NAA 0.2 mg/L and KT 0.1-0.2 mg/L, the rate of callus was noticeably higher than that of NAA 0.2 mg/L and 6-BA 1-3 mg/L. The preferable medium which roots could be induced was 1/2 MS + NAA 0.2 mg/L. |
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| Keywords: | Chrysanthemum morifolium Stem apex Tissue culture |
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