Amplisensor-聚合酶反应定量检测脑脊液中结核分支杆菌DNA的研究

目的　探讨Amplisensor-聚合酶反应 (Amplisensor-PCR)定量检测脑脊液中结核分支杆菌DNA(TB-DNA)对结核性脑膜炎的诊断价值。方法 采用Amplisensor-PCR对117例结核性脑膜炎患者及36例非结核性脑膜炎患者的脑脊液标本进行检测，并与PCR(凝胶电泳后，经溴化乙锭染色)、涂片、培养法比较。结果 Amplisensor-PCR的敏感性显著高于涂片及培养，阳性率分别为57.13%、1.7%、6.7% (P＜0.001)。结论 Amplisensor-PCR可以通过标准曲线划定检出下限，并可换算出标本中原始的靶DNA值，同时具有较高的特异性和敏感性，对结核性脑膜炎的诊断有一定的临床意义。

Detection of DNA of Mycobacterium tuberculosis in cerebrospinal fluid by Amplisensor-PCR

Objective To study the diagnostic value in patients with tuberculous meningitis of the detection of DNA of Mycobacterium tuberculosis in cerebrospinal fluid by Amplisensor-PCR.Methods The DNA of Mycobacterium tuberculosis in cerebrospinal fluid in 117 patients with tuberculous meningitis and 36 patients with non-tuberculous menigitis was detected by Amplisensor-PCR. The result was compared with the data detected by PCR, smear and culture.Results The positive rate of Amplisensor-PCR, smear and culture were respectively 57.3%, 1.7%and 6.7%; The sensitivity of Amplsensor-PCR was significantly high than that of smear and culture.( P <0.001). Conclusion The lower limit of Amplisensor-PCR may be calculated and the value of primitive target DNA be covered through the standard curve. The Amplisensor-PCR had high specificity and sensitivity and diagnostic value to tuberculous meningitis.