安佳欣胶囊对抑郁症模型大鼠基因功能类表达影响的初步研究

目的研究安佳欣胶囊对抑郁症模型大鼠基因功能类表达的影响。方法分析抑郁症大鼠模型组和安佳欣胶囊组各8例样本的基因表达谱数据,筛选差异表达基因。利用基因功能分类体系Gene Ontology中的生物学过程子树和细胞组分子树,寻找和分析显著聚集差异表达基因的复合功能类,从分子水平和基因功能模块水平探索安佳欣胶囊对抑郁症模型大鼠基因表达谱的影响。结果筛选出330个差异表达基因,并进一步识别了8个差异表达基因功能模块,主要涉及糖代谢、蛋白转运、谷氨酰胺代谢、凋亡诱导和神经发生。通过文献证实进一步发现了差异功能模块中7个可能与抑郁症发病相关的基因(Pfkm、Gpx1、Stx1a、Ninj2、Plp、Evl、Nrn1)在两组动物中差异表达。结论安佳欣胶囊可能增加抑郁症模型大鼠的单胺递质合成和释放,改善神经保护和神经发生功能,这些改变可能与安佳欣胶囊的抗抑郁作用有关。

A preliminary study on gene functional expression changes derived from ANJIAXIN capsule in the treatment of depression model rats

Objective To investigate the effect of ANJIAXIN capsule treatment gene expression profiles and gene functional modules of depression model rats. Methods Gene expression profiles were measured in 8 ANJIAXIN-treated depression model rats and 8 control model rats. Differentially expressed genes were identified and annotated to biological process and cellular component categories of Gene Ontology (GO) database. Then, GO categories enriched with significantly differentially expressed genes were identified to investigate the effect of ANJIAXIN capsule treatment at molecular level and gene functional module level. Results Three hundred and thirty differentially expressed genes were identified. Furthermore, eight gene functional modules with significantly gathered differentially expressed genes were recognized, which were mainly related to glycometabolism, protein transport, glutamine metabolism, induction of apoptosis and neurogenesis. Seven differentially expressed genes (Pfkm, Gpx1, Stx1a, Ninj2, Plp, Evl, Nrn1) in the functional modules, which were potentially related to depression, were further identified through literature evidences. Conclusions The effect of ANJIAXIN capsule treatment might be coordinately associated with enhancement of monoamine synthesis and release, and with improvement of neuroprotection and neurogenesis, which suggested the anti-depression effect of ANJIAXIN capsule.