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| 电离辐射对胃癌细胞增殖和周期的影响 | |
| 引用本文： | 高玲,李峰生,陈肖华,刘青杰. 电离辐射对胃癌细胞增殖和周期的影响[J]. 癌变．畸变．突变, 2011, 23(2): 119-122. DOI: 10.3969/j.issn.1004-616x.2011.02.009 |
| 作者姓名： | 高玲 李峰生 陈肖华 刘青杰 |
| 作者单位： | 1. 中国疾病预防控制中心辐射防护与核医学安全所,北京,100088;军事医学科学院放射与辐射医学研究所,北京,100850 2. 第二炮兵总医院,北京,100088 3. 军事医学科学院放射与辐射医学研究所,北京,100850 4. 中国疾病预防控制中心辐射防护与核医学安全所,北京,100088 |
| 摘 要： | 目的：研究电离辐射对体外胃腺癌细胞增殖、凋亡及周期的影响。方法：~（60）Coγ射线单次照射胃腺癌BGC-823细胞,照射剂量分别为0、2、4 Gy,利用MTT和克隆形成率实验检测各剂量组BGC-823的增殖能力;同时利用Annexin-V/PI双染法检测各剂量组BGC-823细胞的凋亡;用PI单染测定各剂量组BGC-823的细胞周期。结果：与0 Gy组相比,2、4Gy 60Coγ射线照射可显著抑制胃腺癌BGC-823细胞的增殖（P〈0.05）,诱导BGC-823细胞在照射后依次出现S期和G2/M期阻滞。4Gy 60Coγ射线照射后48h,凋亡细胞比例显著高于对照组（P〈0.05）。结论：60Coγ射线照射可抑制胃腺癌细胞BGC-823增殖、诱导胃癌细胞出现周期阻滞和细胞凋亡。 |
| 关 键 词： | 胃癌 辐射 增殖 凋亡 细胞周期 |
Effects of ionizing radiation on the proliferation and cell cycle of gastric adenocarcinoma cells |
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| GAO Ling,LI Feng-sheng,CHEN Xiao-hua,LIU Qing-jie. Effects of ionizing radiation on the proliferation and cell cycle of gastric adenocarcinoma cells[J]. Carcinogenesis,Teratogenesis and Mutagenesis, 2011, 23(2): 119-122. DOI: 10.3969/j.issn.1004-616x.2011.02.009 | |
| Authors: | GAO Ling LI Feng-sheng CHEN Xiao-hua LIU Qing-jie |
| Affiliation: | GAO Ling~(1,2),LI Feng-sheng~3,CHEN Xiao-hua~2,LIU Qing-jie~(1,*) (1.National Institute for Radiological Protection,China CDC,Beijing 100088,2.Institution of Radiate Medicine,Academy of Military Medical Sciences,Beijing 100850,3.General Hospital of Second Artillery,China) |
| Abstract: | OBJECTIVE:To study the effects of ionizing radiation on the proliferation,apoptosis and cell cycle of gastric adenocarcinoma cells in vitro.METHODS:After gastric adenocarcinoma BGC-823 cells were irradiated by 60Coγ-rays with a single dose of 0,2 or 4 Gy,the proliferation ability of BGC-823 cells in every dose group was evaluated by MTT and cloning efficiency assay.Apoptosis and cell cycle were further investigated by flow cytometry with Annexin-V/PI double staining and PI staining,respectively.RESULTS:Compared with the 0 Gy group,proliferation of gastric adenocarcinoma cells was inhibited significantly after doses of 2 and 4 Gyγ-rays irradiation（P<0.05）. Furthermore,the cells were arrested in S then G2/M phases afterγ-rays irradiation of 2 and 4 Gy.Finally,compared with 0 Gy group,the percentage of apoptotic cells in 4 Gy dose group increased significantly 48 h after irradiation（P<0.05）. CONCLUSION:Radiation treatment of gastric adenocarcinoma BGC-823 cells with 60Coγ-rays could inhibit cell proliferation,induce cell cycle arrest and promote apoptosis. |
| Keywords: | gastric adenocarcinoma radiation proliferation apoptosis cell cycle |
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