919糖浆抗鸭乙型肝炎病毒的实验研究

目的:研究919糖浆时鸭乙型肝炎的体内抗病毒作用。方法:以感染鸭乙型肝炎病毒(DHBV)的武汉麻鸭为实验动物模型。使1d龄武汉麻鸭感染DHBV,7d后用Dot-EIA法筛选出DHBsAg强阳性鸭。随机分组后给予919糖浆治疗14d,于感染后第7d(为用药前)、用药7d、14d及停药后3d分别采血,采用斑点杂交方法检测血清DHBVDNA。结果。用药919糖浆7d、14d鸭血清DHBV DNA OD值均明显低于给药前,有显著性差异(P<0.05,P<0.01)。停药3d后没有反跳现象。结论:919糖浆有一定的抑制DHBV DNA复制作用。

Experimental Study of 919 Syrup on Duck Hepatitis B Virus in Ducklings

Objective: To study the antiviral effect of 919 syrup on duck hepatitis virus (DHBV) in vivo. Methods: Wuhan brown spot ducklings infected with DHBV were used as the hepatitis B animal model, for which, 1-day old ducklings' were infected with DHBV. Positive DHBV DNA in ducklings', sera were detected by Dot-El A 7 days after the infection, and then 919 syrup was given for 14 days and DHBV DNA was detected by dot-blot hybridization test. Results: After medicated with 919 syrup (125ml/kg d-1) and (62. 5ml/kg d-1) 7 days and 14 days, optic density value (OD value )of DHBV DNA in ducklings' sera were obviously lower than that before treatment, there was significant difference and rebound phenomenon didn't appeared in both groups 3 days after stopping medication (P<0. 05). Conclusion.- 919 syrup could inhibit the replication of DHBV DNA in Wuhan ducklings.