| P38MAPK通路在BMP9诱导C3H10T1/2干细胞向心肌样细胞分化中的作用 |
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| 引用本文： | 张芬,陈沅,孙文静,陈露,陈妙月,朱高慧.P38MAPK通路在BMP9诱导C3H10T1/2干细胞向心肌样细胞分化中的作用[J].重庆医科大学学报,2012(8):657-660. |
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| 作者姓名： | 张芬 陈沅 孙文静 陈露 陈妙月 朱高慧 |
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| 作者单位： | 重庆医科大学附属儿童医院心血管内科 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30772361) |
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| 摘 要： | 目的:研究P38丝裂原活化蛋白激酶(P38mitogen-activated protein kinases,P38MAPK)通路在骨形态发生蛋白9(Bonemorphogenetic protein 9,BMP9)诱导C3H10T1/2干细胞向心肌样细胞分化中的作用。方法:免疫印迹检测经pAdEasyBMP9诱导24 h的C3H10T1/2干细胞磷酸化P38MAPK和P38MAPK表达水平,免疫荧光定位磷酸化P38MAPK;SB203580抑制C3H10T1/2干细胞磷酸化P38MAPK活性,pAdEasyBMP9诱导21 d时免疫印迹检测心肌特异性蛋白CX43、cTnT表达,免疫荧光检测心肌特异性蛋白cTnT、α-MHC表达,RT-qPCR检测心肌特异性基因GATA4、MEF2C表达。结果:pAdEasyBMP9促进磷酸化P38MAPK表达增高,却不影响P38MAPK表达水平;磷酸化P38MAPK有表达于胞浆和胞核、仅表达于胞核的不同状态。SB203580可显著性地抑制由pAdEasyBMP9诱导的C3H10T1/2干细胞CX43、cTnT、α-MHC、GATA4、MEF2C表达。结论:pAdEasyBMP9诱导C3H10T1/2干细胞能激活P38MAPK通路,并促进其向心肌样细胞分化。
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| 关 键 词： | 骨形态发生蛋白9 C3H10T1/2干细胞 心肌样细胞 P38丝裂原活化蛋白激酶 |
Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes |
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| ZHANG Fen,CHEN Yuan,SUN Wenjing,CHEN Lu,CHEN Miaoyue,ZHU Gaohui.Role of P38 mitogen-activated protein kinases signaling pathway in the differentiation of bone morphogenetic protein 9 induced C3H10T1/2 stem cell into myocardiocytes[J].Journal of Chongqing Medical University,2012(8):657-660. |
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| Authors: | ZHANG Fen CHEN Yuan SUN Wenjing CHEN Lu CHEN Miaoyue ZHU Gaohui |
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| Institution: | (Department of Cardio1ogy,the Children’s Hospital,Chongqing Medical University) |
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