| IL—2／LAK细胞对麻风杆菌感染巨噬细胞的溶解作用 |
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| 引用本文： | 顾立刚 Krah.,JL. IL—2／LAK细胞对麻风杆菌感染巨噬细胞的溶解作用[J]. 中华微生物学和免疫学杂志, 1995, 15(4): 234-237 |
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| 作者姓名： | 顾立刚 Krah. JL |
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| 作者单位： | 北京中医药大学微生物学教研室，美国国家汉森氏疾病防治中心免疫研究室 100029 |
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| 基金项目： | 美国Heiser Program for Research in Leprosy Postdoctoral Fellow资助 |
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| 摘 要： | 观察了小鼠IL-2/LAK细胞体外对麻风杆菌感染巨噬细胞(Mφ)的溶解作用。结果显示,当麻风杆菌感染Mφ比率在1:1,10:1和50:1时,经体外培养1,3和5天后,效应细胞与靶细胞比例在10:1时,对麻风杆菌感染Mφ比率在10:1和50:1的靶细胞溶解作用比没有感染的Mφ或麻风杆菌与Mφ比率1:1的靶细胞显著增加。而且溶解百分率随着培养时间增加而提高。用放射性同位素标记麻风杆菌的代谢活力测定发现,LAK细胞能抑制麻风杆菌氧化(14)~C-棕榈酸产生(14)~CO_2。提示IL-2/LAK细胞在溶解麻风杆菌感染的巨噬细胞时,可能还具有影响胞内杀菌物质对麻风杆菌活力代谢的作用。
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| 关 键 词： | 麻风杆菌 细胞毒 白细胞介素2 |
LYSIS EFFECT OF IL-2/LAK CELLS AGAINST MYCOBACTERIUM LEPRAE-INFECTED MACROPHAGE |
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| Gu Ligang,James L. Krahenbuhl. LYSIS EFFECT OF IL-2/LAK CELLS AGAINST MYCOBACTERIUM LEPRAE-INFECTED MACROPHAGE[J]. Chinese Journal of Microbiology and Immunology, 1995, 15(4): 234-237 |
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| Authors: | Gu Ligang James L. Krahenbuhl |
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| Affiliation: | Gu Ligang,James L. KrahenbuhlDepartment of Microbiology,Beijing University ofTraditional Chinese Medicine,Beijing 100029 |
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| Abstract: | Lysis effect of IL-2/LAK cells against M. leprae-infected M in vitro were observed. The results showed that IL-2/LAk cells against M. leprae-infected M at M.. leprae : M ratios of 1 : 1,10:Land 50 :1,after infected M incubation for one,three and five days,the lysis of M. leprae-infected M at M. leprae : M 10 : 1 and 50 : 1 were significantly greater than of uninfected M or M infected with M. leprae at ratio of 1 : 1 at E : T ratio of 10 : 1. A sensitive radiorespirometric assay was employed to measure the metabolism/viability of M. leprae released from M by LAK cells. IL-2/LAK cells could inhibit the metabolic capacity of M. leprae to oxidize 14C- palmitic acid to 14CO2. This suggests that IL-2/LAK cells may be used as a immunotherapy of diseases caused by intracellular pathogens. |
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| Keywords: | M ycobacterium leprae IL-2/LAK cell (14)~C palmitic acid Cytotoxicity |
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