硒对LAK细胞活性的影响及其机理研究

研究了硒在体外对LAK细胞活性的影响及作用机理,结果证明在LAK细胞的诱导阶段加入10~5～10mol/L亚硒酸纳能增强LAK细胞的杀伤和增殖活性。采用流式细胞仪测Tac(IL—2Rα)表达,Slot-Blot检测硒对LAK细胞的IL—2RαmRNA水平的影响,结果表明硒能增强LAK细胞的Tac抗原表达和IL-2RαmRNA的水平,提示硒可能通过促进Tac的表达增强了LAK细胞对IL—2的敏感性,从而提高了LAK细胞的增殖及杀伤活性。因此硒可望作为一种新型的免疫调节剂用于抗肿瘤治疗。

STUDY ON THE EFFECT OF SELENIUM ON LAK CELL ACTIVITY AND ITS POSSIBLE MECHANISMS

The effect of selenium on lymphokine-activated killer cell(LAK) activity and the possible mechanisms were studied. The proliferation and cytotoxic activity of LAK cells was enhanced in the presence of Se in the culture medium during LAK cell induction. The effect of selenium on the expression of Tac was assayed by Flow Cytometry and IL-2Ra mRNA expression of LAK cells was detected by Slot Blot analysis. An increase of Tac and lL-2Ra rnRNA expression in the presence of selenium in the culture medium was observed. The data suggest that the augmentory effect of selenium on the proliferation and cytotoxicity of LAK cells induced with rIL-2 might partially mediated by the enhancement of Tac expression. The data also revealed the possibility of using selenium as a new immunomodulator in cancer immunotherapy.