建立人微小病毒B19的PCR检测方法

人微小病毒Ｂ１９是微小病毒科中唯一能感染人的病毒。能够提供Ｂ１９抗原的病毒体外培养系统尚未建成，因此限制了血清学实验的开发和普及。为此作者建立起Ｂ１９病毒的ＰＣＲ检测方法。设计引物在表达外壳蛋白ＶＰ１基因区，扩增长度为４００ｂｐ。

ESTABLISHMENT OF PCR FOR DETECTING HUMAN PARVOVIRUS B19

Of several parvoviruses.only the human parvovirus B 19 has been shown to be a human pathogen. Culture systems producing human parvovirus B19 have not been developed in vitro. Consequently,sero diagnostic tests for this infection are not widely available. So we have tried to use PCR technique to delect B19. The designed primers were in the con servative sequences which express the VP1 capsid protein. The experiments of sensitivity .specificity and identification of the amplified products have been carried out for this pair of primers. The results showed that: lfg B19 DN A could be detected ;only amplified products of BI9 could be viewed under UV light,and none of amplifited products of human genome,HBV ,HPV ,HSV ,HCMV ,and ADV could be found. The amplifited products have been identified to be the target DNA ,using Hae III I ,Pst I and Sty I digestions. This method was highly sensitive and specific. It has laid a foundation for investigation of B19 in China.