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| 环磷酰胺诱导小鼠HAC肝癌细胞凋亡和抑制增殖的体内实验研究 | |
| 引用本文： | 周振华,宋明志,于尔辛. 环磷酰胺诱导小鼠HAC肝癌细胞凋亡和抑制增殖的体内实验研究[J]. 肿瘤, 2000, 20(3): 161-163,F003 |
| 作者姓名： | 周振华 宋明志 于尔辛 |
| 作者单位： | [1]上海医科大学肿瘤医院中医科 [2]病理科 |
| 摘 要： | 目的 探讨环磷酰胺对荷瘤鼠ＨＡＣ肝癌细胞凋亡和增殖的影响。方法 建立小鼠ＨＡＣ肝癌荷瘤模型，用ＴＵＮＥＬ法和免疫组化法检测环磷酰胺作用下，ＨＡＣ肝癌细胞的凋亡和ＰＣＮＡ表达的变化。结果 １５ｍｇ／ｋｇ环磷酰胺的抑瘤率４３％。ＨＡＣ肝癌细胞的凋亡指数１．９３％，较荷瘤对照组１．１１％明显升高（Ｐ〈０．０５）。增殖指数３５．７５％，较荷瘤对照组５６．３８％明显下降（Ｐ〈０．０１）。结论 环磷酰胺有诱导 |
| 关 键 词： | 环磷酰胺 细胞凋亡 细胞增殖 肝肿瘤 |
INVIVO STUDY ON INDUCING APOPTOSIS AND REDUCING CELL PROLIFERATION BY CYCLOPHOSPHAMIDE INMICE WITH HAC LIVER CANCER |
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| ZHOU Zhenhua,SONG Minzhi,YU Erxin,et al Cancer Hospital,Shanghai Medical University,Shanghai ,China. INVIVO STUDY ON INDUCING APOPTOSIS AND REDUCING CELL PROLIFERATION BY CYCLOPHOSPHAMIDE INMICE WITH HAC LIVER CANCER[J]. Tumor, 2000, 20(3): 161-163,F003 | |
| Authors: | ZHOU Zhenhua SONG Minzhi YU Erxin et al Cancer Hospital Shanghai Medical University Shanghai China |
| Affiliation: | ZHOU Zhenhua,SONG Minzhi,YU Erxin,et al Cancer Hospital,Shanghai Medical University,Shanghai 200032,China |
| Abstract: | Objective To observe the apoptosis and cell proliferation induced by cyclophosphamide in mice liver cancer. Methods Therapeutic effect of 15 mg/kg cyclophosphamide on mice HAC liver cancer was observed in vivo. Apoptosis and PCNA of tumor cells were also studied by using TUNEL and immunohistochemical methods. Results In the group of control: the apoptotic cell index (AI) in tumor cell was 1.11%. PCNA index (PI) was 56.38 %. In the group of CTX: the rate of tumor inhibition was 43 %; the AI was 1.93 %, represented a significant increase (P<005); the PI was 35.75 %, a statistically significant change (P<001).Conclusion Cyclophosphamide may induce apoptosis and may reduce cell proliferation.$$$$ |
| Keywords: | Cyclophosphamide Tumor cells cultured Apoptosis Cell proliferation |
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