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| HPLC法测定连蒲双清片中盐酸小檗碱的含量 | |
| 引用本文： | 周家才,梁玉梅. HPLC法测定连蒲双清片中盐酸小檗碱的含量[J]. 安徽医药, 2008, 12(12): 1150-1151 |
| 作者姓名： | 周家才 梁玉梅 |
| 作者单位： | 周家才 (南京医药合肥天星有限公司,安徽,合肥,230061)； 梁玉梅 (南京医药合肥天星有限公司,安徽,合肥,230061)； |
| 摘 要： | 目的采用高效液相色谱法测定连蒲双清片中盐酸小檗碱的含量。方法Kromasil C18色谱柱（250 mm×4.6 mm,5μm）;乙腈-0.05 mol.L^-1磷酸二氢钾溶液（30∶70）为流动相,流速为1.0 ml.min^-1;检测波长为347 nm。结果盐酸小檗碱线性范围为8.0^127.2 mg.L^-1（r=0.9991,n=5）,平均回收率为99.74%,RSD=0.54%（n=6）。结论该方法准确、简便,可用于该制剂的质量控制。 |
| 关 键 词： | 高效液相色谱法 盐酸小檗碱 连蒲双清片 HPLC法 测定 |
Determination of berberine in lianpushuangqing tablets by HPLC |
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| ZHOU Jia-cai,LIANG Yu-mei. Determination of berberine in lianpushuangqing tablets by HPLC[J]. Anhui Medical and Pharmaceutical Journal, 2008, 12(12): 1150-1151 | |
| Authors: | ZHOU Jia-cai LIANG Yu-mei |
| Affiliation: | ( Nanjing Medical Hefei Tianxing Co, Ltd, Hefei 230061 ) |
| Abstract: | Aim To establish an HPLC method for determining berberine in lianpushuangqing tablets. Method C18 .column（250 mm × 4.6 mm,5 um） was used and the mobile phase was composed of acetonitrile-0.05 mol · L^-1 KDP （30： 70）. The flow rate was 1. 0 ml · min^ - 1. The detection wavelength was at 347 nm. Results The linear range of berberine was from 8.0 to 127.2 mg!· L ^- 1 （ r = 0. 999 1, n = 5） and the average recovery was 99.74%, with RSD being 0.54% （ n = 6）. Conclusion The method is accurate and simple for quality control of the preparation. |
| Keywords: | HPLC berberine lianpushuangqing tablets |
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