电离辐射诱发人外周血miRNA-150表达水平的改变及意义

目的 通过实时定量PCR技术检测不同剂量照射引起人外周血miRNA-150表达的差异，从而探讨miRNA-150在机体辐射损伤中的意义。方法 将采集的静脉血分别给予0.5、1、2、4、8 Gy剂量照射，并设置对照组。照射后将血样接种于含10%小牛血清的RPMI1640培养瓶中，置于37℃恒温培养箱中培养，利用实时定量PCR技术检测照射8、24 h后miR-150的含量变化；采用tbools软件进行miR-150序列比对，Starbase数据库预测miR-150靶基因。结果 miR-150在0.5、1、2、4、8 Gy照射后8 h后2^-△△Ct值分别为0.66、0.68、0.74、0.75、0.63，与未照射组相比，差异有统计学意义；照射24 h后2^-△△Ct值分别为0.94、0.95、0.71、0.59、0.67，较8 h均出现了不同程度的表达下调，与未照射组相比，差异有统计学意义；人与小鼠miR-150-5p序列一致，miR-150-3p种子序列区存在单一碱基差异，靶基因预测表明BASP1、MAP3K12和ZBTB4基因为人miR-150靶向基因的可能性最高。结论 照射后外周血中miR-150差异表达与辐射损伤有关，可成为预测辐射损伤的潜在生物标志物。

Ionizing radiation induced miRNA-150 changes in peripheral blood

Objective To study the differential regulations of miRNA-150 in human peripheral blood after ionizing radiation.Methods Venous blood was collected and exposed to 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy X-ray and set a control group at the same time. Blood samples were inoculated in culture bottles containing 10% calf serum and cultured in 37℃ incubators after radiation.miRNA-150 changes were detected after 8 and 24 hours post radiation by PCR. Sequence alignment for miRNA150 use tbools software. Predict the target gene of miRNA150 use Starbase datebase.Results After 8 h with 0.5 Gy、1 Gy、2 Gy、4 Gy、8 Gy radiation,miRNA-150 expression levels were 0.66、0.68、0.74、0.75、0.63, compared with the unirradiated group, the results were statistically significant; miRNA-150 expression levels were 0.94、0.95、0.71、0.59、0.67 after 24 h, the expression was downregulated compared to 8 h, compared with the unirradiated group, the results were statistically significant. Human and mouse have the same sequence in miRNA-150-5p. There is a single base difference in the Seed sequence region of miRNA-150-3p. The target gene prediction shows that there is a highest probability of BASP1,MAP3K12 and ZBTB4 as the target genes of miRNA-150 for human.Conclusion The expression of miRNA-150 in peripheral blood after radiation is related to radiation injury and has a potential serum marker for radiation injury.