The products of photoreversing rhodopsin bleaching by microsecond flashes in the isolated vertebrate retina

Bleaching experiments were carried out at room temperature on rhodopsin in isolated rat, frog and rat retinas with blue and orange laser flashes of very high energy and duration less than 3 microseconds. Blue flashes bleached a maximum of about 50% at the highest energies; orange ones bleached about 30% at intermediate energies but the value decreased to below 20% as the energy increased. This bleaching behaviour can be explained in terms of a kinetic model which assumes that bleaching is photoreversed during the flashes and which incorporates the relevant properties of rhodopsin, isorhodopsin, bathorhodopsin and lumirhodopsin.