肝癌组织及血清中细胞间粘附分子-1表达的研究

目的研究细胞间粘附分子－１（ｉｎｔｅｒｃｅｌｕｌａｒａｄｈｅｓｉｏｎｍｏｌｅｃｕｌｅ－１，ＩＣＡＭ－１）作为判断肝癌发展程度及转移状态指标的可能性。方法以免疫组化的方法检测ＩＣＡＭ－１在肝癌组织和正常肝组织中的表达，观察了ＩＣＡＭ－１表达在细胞的定位。以点免疫印迹法测定不同患者血清和不同肝组织中ＩＣＡＭ－１的表达。分析ＩＣＡＭ－１表达与肿瘤生长转移状态、肿瘤特性的关系。结果肝癌细胞ＩＣＡＭ－１表达阳性（阳性率为８００％），主要分布在细胞膜，正常肝细胞则为阴性。肝癌患者血清可溶性细胞间粘附分子－１（ｓＩＣＡＭ－１）水平高于正常人（Ｐ＜００１）及肝良性肿瘤患者（Ｐ＜００５），肝癌伴转移者高于无转移者（Ｐ＜００５）。肝癌组织中ＩＣＡＭ－１含量明显高于癌旁组织（Ｐ＜００１）及正常肝组织（Ｐ＜００１），而与肿瘤大小及有无包膜无关（Ｐ＞００５）；转移组肝癌中ＩＣＡＭ－１的表达明显高于非转移组（Ｐ＜００５），两组癌旁组织中ＩＣＡＭ－１表达无差别（Ｐ＞００５）。结论血清及组织中ＩＣＡＭ－１的水平在一定程度上可以反映肝癌发展程度及转移状态，有可能作为预测肝癌转移复发的指标。

STUDY ON THE RELATIONSHIP BETWEEN INTERCELLULAR ADHESION MOLECULE-1 AND LIVER CANCER METASTASIS

Objective To study the relationship between intercellular adhesion molecule-1 (ICAM-1) and liver cancer metastasis and to search an index that can reflect the growth and the metastasis of liver cancer. Methods ICAM-1 expression in fresh tissue of normal liver and liver cancer was examined by immunoperoxidase staining. Serum soluble intercellular adhesion molecule-1 (sICAM-1) from patients with liver cancer, benign tumor and normal control, and the expression of ICAM-1 in hepatoma, tumor surrounding liver and normal liver were semi-quantitatively analyzed by Dot immuno-blot. Results The expression of ICAM-1 was positive (80 0%, 36/45) in the cell of hepatoma, meanwhile was negative in normal. SICAM-1 content was the highest in patients with liver cancer comparing with that in patients with benign tumor (P<0 05) or normal control(P<0 01), and was higher in patients with metastasis than those without metastasis (P<0 05). Tissue ICAM-1 was higher in liver cancer than in tumor surrounding liver (P<0 01) and in normal tissue (P<0 01), and was also higher in liver cancer with metastasis than in liver cancer without metastasis(P>0 05). Conclusion ICAM-1 could be a marker of the stage and metastasis of liver cancer and may play a role in the biological character of hepatoma cell.