美沙拉嗪对溃疡性结肠炎患者肠黏膜紧密连接蛋白表达的影响

背景:溃疡性结肠炎(UC)是一种较常见的消化道疾病,其病因和发病机制尚未完全阐明,治疗缺乏特异性方法,导致病情迁延反复。目的:探讨美沙拉嗪治疗对UC肠道紧密连接蛋白的影响。方法:选取2010年12月～2011年6月南京医科大学附属南京医院收治的活动期UC患者30例,给予美沙拉嗪1.0 g tid治疗8周。治疗前后患者行结肠镜检查取活检,以免疫组化法检测治疗前后紧密连接蛋白occludin、ZO-1的表达。结果:美沙拉嗪治疗后UC患者内镜下均取得缓解。免疫组化结果显示,治疗后UC肠黏膜上皮细胞中occludin蛋白(7.20±1.55对1.60±0.55)、ZO-1蛋白表达(6.60±1.26对1.40±0.55)显著高于治疗前,差异均有统计学意义(P<0.05)。结论:美沙拉嗪可能通过上调紧密连接蛋白occludin、ZO-1的表达来保护肠黏膜屏障功能。

Effect of Mesalazine on Expressions of Intestinal Tight Junction Proteins in Ulcerative Colitis

Background： Ulcerative colitis （UC） is a commonly seen digestive disease, its etiology and pathogenesis are not yet fully elucidated. The lack of specific treatment results in repeated and persistent disease course. Aims： To investigate the effect of mesalazine on the expressions of intestinal tight junction proteins in UC. Methods： Thirty active UC patients admitted from December 2010 to June 2011 at Nanjing First Hospital Affiliated to Nanjing Medical University were enrolled. Mesalazine 1.0 g tid was given for 8 weeks. Colonoscopy was performed before and after mesalazine treatment. Expressions of occludin and ZO-1 were determined by immunohistochemistry. Results： After mesalazine treatment, all the UC patients were at remission stage under endoscopy. Expressions of occludin （7.20 -＋ 1.55 vs. 1.60 ＋0.55） and ZO-1 （6.60 ± 1.26 vs. 1.40 ±0. 55） after mesalazine treatment were significantly higher than those before mesalazine treatment （ P 〈 0.05 ）. Conclusions ： Mesalazine may protect the intestinal mucosa barrier through the up-regulation of expressions of intestinal tight junction proteins.