Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid |
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| Authors: | AD C.J.M. Antonissen Kok P.M. Van Kessel Hans Van Dijk Jan M.N. Willers |
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| Affiliation: | Section of Immunology, Laboratory of Microbiology, State University of Utrecht, Catharijnesingel 59, 3511 GG Utrecht, The Netherlands |
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| Abstract: | ![]()
A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose. |
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| Keywords: | To whom correspondence should be sent. |
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