VEGF启动子介导双自杀基因重组腺病毒对LoVo细胞的体外杀伤作用

目的 探讨VEGF启动子驱动的CD／TK双自杀基因重组腺病毒(Ad-VEGFp-CDglyTK)对大肠癌LoVo细胞的体外杀伤作用。方法 分别用重组腺病毒Ad-VEGFp-CDglyTK、Ad-CMV-CD、Ad-CMV-TK、Ad-CMV-CDglyTK转染大肠癌LoVo细胞，给予前药5-FC、GCV，测定LoVo细胞的集落形成、细胞存活率及该治疗系统的旁观者效应。结果 与Ad-CMV-CD、Ad-CMV-TK相比，Ad-VEGFp-CDglyTK系统对LoVo细胞的集落形成、细胞生长均具有更强抑制作用，并可见较明显的旁观者效应：而与CMV启动子的双自杀基因相比，其作用无显著性差异。结论 VEGF启动子驱动的双自杀基因治疗系统对大肠癌LoVo细胞有确切的杀伤作用，其作用强度与强启动子CMV驱动的双自杀基因治疗系统似。

In vitro antitumor effect of adenovirus containing CD-TK fusion gene driven by VEGF promoter on LoVo cells]

OBJECTIVE: To evaluate the in vitro antitumor effect of adenovirus containing cytosine deaminase (CD) and thymine kinase (TK) gene driven by vascular endothelial growth factor (VEGF) promoter (Ad-VEGFp-CDglyTK) on human colorectal cancer LoVo cells. METHODS: The adenoviral vectors Ad-VEGFp-CDglyTK, Ad-CMV-CD, Ad-CMV-TK and Ad-CMV- CDglyTK were transfected into LoVo cells, which were then treated with the prodrugs 5-FC or GCV to observe the plating efficiency and growth inhibition effect and bystander effect of the vectors on LoVo cells. RESULTS: Compared with the two vectors containing single suicide gene, Ad-VEGFp-CDglyTK showed more remarkable inhibitory effect on the plating efficiency and growth of the tumor cells, and the bystander effect of the fusion suicide gene was much stronger than that produced by single suicide gene. But the LoVo cells infected with Ad-VEGFp-CDglyTK exhibited similar sensitivity to the prodrugs with the cells infected with Ad-CMV-CDglyTK. CONCLUSION: Ad-VEGFp-CDglyTK has potent antitumor effect on LoVo cells in vitro, which is comparable with the effect of Ad-CMV-CDglyTK.