睡眠剥夺大鼠大脑皮层和海马nNOS表达的改变在其学习记忆损伤中的作用

目的观察睡眠剥夺（SD）后,大鼠学习记忆能力及大脑皮层和海马神经元型一氧化氮合酶（nNOS）蛋白表达的变化。方法采用小平台水环境法建立大鼠SD模型。观察大鼠经过不同时间SD后,各组“Y”迷宫成绩及大脑皮层和海马nNOS阳性神经元的变化情况。结果（1）“Y”迷宫实验：3组SD组的迷宫成绩较正常笼养对照组（CC）分别下降了24．3％、17．6％和41．9％（P〈0．01）。（2）nNOS阳性神经元数目：在皮层,SDld组较CC组增加了8．7％（P〈0．01）,SD2d组和SD3d组较CC组则减少了9．0％和16．0％（P〈0．01）;在海马CA。区,3个SD组较CC组分别减少了9．4％、16．0％和22．0％（P〈0．01）。结论SD可能通过改变大鼠大脑皮层和海马CA．区nNOS蛋白的表达来损害大鼠学习记忆能力。

Role of cortical and hippocampal nNOS protein in memory deficits induced by sleep deprivation in rats

Objective To observe the changes of learning and memory as well as neuronal nitric oxide synathase (nNOS) in cortex and hippocampus of rats after sleep deprivation (SD). Methods SD was induced in male Wistar rats by employing "flower pot" technique. The performance in Y maze and the number of nNOS positive neurons in cortex and hippocampus after different time of SD were measured. Results (1) The correct rates of Y maze test were 24. 3% , 17. 6% , 41. 9% less in 3 SD groups than CC group(P <0. 01). (2) The number of nNOS positive neurons in cortex was 8.7% more in SDld group than CC group(P<0. 01) ; but 9.0% and 16.0% less in SD2d group and SD3d group than CC group(P <0. 01). In hippocampal CA1 sector, nNOS positive neurons of the 3 SD groups were 9. 4% , 16. 0% and 22.0% less in number respectively than CC group. Conclusion SD induces the expression of changes nNOS protein in cortex and hippocampal CA1 and thereby impairs learning and memory in rats.