液相-电喷雾离子化-质谱在DNA加合物检测中的应用进展

DNA加合物是一种判断DNA损伤的重要的生物标志物。对于某些特殊DNA加合物的检测不仅能够获取个体对于这些化合物的接触情况,而且还可以判断其可能产生的效应。DNA加合物有许多检测方法,如32P后标记法、免疫法和液相/气相-质谱联用法等等。随着液相-质谱偶联技术(LC-MS)的发展,离子化方法尤其是电喷雾离子化(ESI)方法在技术上的突破以及离子发射和检测技术的进步,LC-MS可以检测暴露外源遗传毒性化合物后与动物和人组织DNA形成的加合物水平。本文就LC-ESI-MS液相质谱联用技术在检测碱基、核苷和核苷酸DNA加合物方面的应用做一简述。

Progress in application of liquid-chromatogram-electrospray ionization-mass spectrum in determination of DNA adduct

DNA adducts are an important biomarker predicting DNA damage. Many determination methods have been developed since the discovery of DNA adducts, such as ³²P-post labeling, immunological method, liquid chromatography/gas chromatography-mass spectrometer. Recently, with the development of liquid chromatography coupled with mass spectrometer (LC-MS), especially breakthroughs in electrospay ionization (ESI) and progress in mass detection, it is feasible to determine the DNA adducts levels in animal and human tissues after exposure to genetic carcinogens. This paper summarizes the applications of LC-ESI-MS to the determination of base-, nucleoside- and nucleotide-adducts.