活血解毒方对糖尿病早期大鼠视网膜功能的影响

目的 观察活血解毒方对糖尿病早期大鼠模型视网膜电图(electroretinogram, ERG)和大鼠视网膜组织胶质纤维酸性蛋白(glial fibrillary acid protein, GFAP)表达的影响。方法 采用链脲佐菌素(65 mg/kg)一次性腹腔注射SD大鼠建立糖尿病大鼠模型，随机分为模型组和活血解毒方低(3.85 g/kg)、中(7.70 g/kg)、高剂量组(15.40 g/kg)及西药组(羟苯磺酸钙胶囊，0.167 g/kg)，每组8只。另设正常对照组(8只，给予等体积蒸馏水灌胃)，各组均灌胃给药20周。测定ERG，检测暗适应眼最大电反应的a、b波振幅及振荡电位(oscillatory potentials, OPs)振幅总和。并采用免疫组织化学法、Western blot和荧光定量PCR法检测大鼠视网膜积分光密度值(integral optical density, IOD)、GFAP蛋白及 mRNA表达。结果 与正常组比较，模型组大鼠ERG a波、b波和OPs振幅降低(P<0.01), IOD、GFAP蛋白及mRNA表达升高(P<0.01)。与模型组比较，活血解毒方各剂量组b波和OPs振幅升高, IOD、GFAP蛋白及mRNA表达降低，活血解毒方中、高剂量组a波振幅值升高；西药组b波振幅值升高, IOD表达降低，差异均有统计学意义(P<0.05, P<0.01)。西药组与活血解毒方各剂量组各指标比较，差异无统计学意义。结论 活血解毒方可减轻糖尿病早期大鼠视觉电生理功能损害，对视网膜神经胶质细胞有一定的保护作用。

Effects of Huoxue Jiedu Recipe on Retinopathy in Early Diabetic Rats

Objective To observe the effects of Huoxue Jiedu Recipe(HJR) on the electroretinogram(ERG) and the expression of glial fibrillary acid protein(GFAP) in the retina tissue of early diabetic rats.MethodsThe diabetic rat model was established using one single intraperitoneal injection of streptozotocin(STZ,65 mg/kg).Then the modeled rats were randomly divided into 5 groups,i.e.,the model group,the low dose HJR group(3.85 g/kg),the middle dose HJR group(7.70 g/kg),the high dose HJR group(15.40 g/kg),and the Western medicine treatment group(Calcium Dobesilate Capsule,0.167 g/kg),8 in each group.A normal control group consisting of 8 rats was also set up,which was given equal volume of distilled water by gastrogavage.All rats were medicated by gastrogavage for 20 weeks.The electroretinograph(ERG) was determined.The amplitudes of wave a and b(the maximal electric reaction for dark-adapted eyes),and the amplitude sum of the oscillatory potentials(OPs) were detected.The integral optical density(IOD),the protein and mRNA expression of GFAP were detected using immunohistochemical assay,Western blot,and fluorescent quantitative PCR.Results Compared with the normal control group,the amplitudes of wave a,wave b,and OPs decreased in the model group(P<0.01).The protein and mRNA expressions of IOD and GFAP significantly increased(P<0.01).Compared with the model group,the amplitudes of wave b and OPs increased,and the protein and mRNA expressions of IOD and GFAP significantly decreased in each HJR group.The amplitude of wave a in the middle and high dose HJR groups increased.The amplitude of wave b increased and the IOD expression decreased in the Western medicine treatment group,showing statistical difference(P<0.05,P<0.01).There was no statistical difference in each index between the Western medicine treatment group and each HJR group.Conclusion HJR could attenuate the visual electrophysiological dysfunction in early diabetic rat,showing certain protection on retinal glial cells.