高效液相色谱法测定熊果酸原料中有关物质含量

摘要 目的建立高效液相色谱（HPLC）法测定熊果酸原料中的有关物质含量，为制定其质量标准提供依据。方法采用Agilent XDB C18 （250 mm×4.6 mm，5 μm）色谱柱，流动相为水相［水：三乙胺：冰醋酸（10：0.03：0.06）］与甲醇系统，梯度洗脱，检测波长210 nm,柱温30 ℃，流速1.0 mL·min－1，进样量20 μL。结果熊果酸中齐墩果酸含量均＜0.5%；其他杂质均未检出。结论该方法可以作为熊果酸原料中的有关物质的检查方法，简便，快速，准确，专属性好。

Determination of Related Substances in Raw Materials of Ursolic Acid by HPLC

Objective To establish a method for determination of related substances of ursolic acid raw materials and provide the evidence for its quality control.Methods The chromatographic column was Agilent XDB-C18(250 mm×4.6 mm,5 μm).The mobile phase consisted of methanol-water phase [water:triethylamine:glacial acetic acid(10:0.03:0.06)].The gradient elution was applied as a chromatographic separation,and the detection wavelength was set at 210 nm.Results The oleanolic acid contained in the ursolic acid was less than 0.5%,and other impurities were not detected.Conclusion This method is simple,rapid,accurate and has a good specificity that can be used in checking the related substances in raw materials of ursolic acid.