Bp3抗耐氟康唑白念珠菌活性研究

目的研究五环三萜类活性单体（saikosaponines,Bp3）的抗耐氟康唑白念珠菌（fluconazole-resistant candida albicans,FRCA）作用及其与氟康唑的协同作用。方法对柴胡进行有效成分分离,并对其抗菌作用明确的成分进行结构测定,确定其分子结构,得到结构明确的单体Bp3,参照（national committee for clinical laboratory standard,NCCLS）标准,测定Bp3对20株FRCA的最低抑菌浓度值（minimal inhibitory concentration,MIC）及其与氟康唑的协同作用。结果联合用药时显著减少了每一药物的MIC值,在20株FRCA均表现为协同或相加作用方式。结论结构明确的五环三萜类单体Bp3对FRCA有一定的抑制作用,且与氟康唑有协同或相加作用。

Activity of monomers of pentaeyclic triterpennoids of Saikosaponines against Fluconazole-resistant Candida albicans

Objective To study the effect of monomers of Pentaeyclic triterpennoids （ Bp3 ） isolated from Bupleurum kunmingense on Fluconazole-resistant Candida albicans （FRCA） synergism of BP3 with Fluconazole. Methods The chemical structure of the antimicrobial activity of isolated monomers from Bupleumm kunmingense is BP3. Minimal inhibitory concentrations （MIC） of Bp3 and synergism with Fluconazole on 20 FRCA strains were detected by microdilution method and checkerboard microdilation method according to the Standard of National Committee for Clinical Laboratory （NCCLS）. Results When Bp3 and Fluconazole were given in combination, the MIC of this combination to FRCA was significantly reduced. Bp3 and Fluconazole showed synergistic effects or additional effects on 20 FRCA strains. Conclusion Bp3 can inhibit FRCA to some extent. The combination effect of Bp3 and fluconazole is significantly more active than any drug alone against FRCA.