| 纤维连接蛋白在形觉剥夺眼和正常眼的差异表达 |
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| 引用本文： | 初歌今,张清炯,成建定,申煌煊,肖学珊,黎仕强.纤维连接蛋白在形觉剥夺眼和正常眼的差异表达[J].眼科研究,2001,19(2):101-103. |
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| 作者姓名： | 初歌今 张清炯 成建定 申煌煊 肖学珊 黎仕强 |
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| 作者单位： | 1. 中山医科大学中山眼科中心 广州,510060
2. 中山医科大学法医病理学教研室 |
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| 基金项目： | 本课题受广东省重点科技攻关项目(99M04805G)、211工程基金(基金编号:98001)资助 |
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| 摘 要： | 目的：探讨实验性形觉剥夺性近视眼眼轴增大的影响因素，探讨导致形觉剥夺性近视眼的巩膜改变的分子水平机制。方法：单眼遮盖20只鸡雏4周制备实验性近视模型，然后使用免疫组织化学方法检测遮盖眼和对照眼的巩膜软骨组织的纤维连接蛋白（FN）表达情况。结果：遮盖眼巩膜软骨的软骨细胞质、细胞膜和软骨囊的FN的表达水平比对照眼明显增高（P＜0.01)。结论：FN与实验性近视眼的近视形成机制有关，可能有调节眼球增大的作用。
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| 关 键 词： | 近视眼 纤维连接蛋白 免疫组织化学 |
| 修稿时间： | 2000年7月19日 |
Differential expression of fibronectin in experimental myopia and normaleyes in chicks |
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| Chu Gejin,Zhang Qingjiong,Cheng Jianding,et al..Differential expression of fibronectin in experimental myopia and normaleyes in chicks[J].Chinese Ophthalmic Research,2001,19(2):101-103. |
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| Authors: | Chu Gejin Zhang Qingjiong Cheng Jianding |
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| Abstract: | Objective To investigate the involvement of fibronectin in the development of experiment form-deprivation myopia. MethodsNewborn chicks were reared in a room which was warmed by non-illuminating heater on a 12 hr light/dark cycle provided by cool fluorescent lighting.The illumination at chick eye level is about 40 Lx.Experimental myopia was established by monocular suture of eyelid for 4 weeks.Myopia of the chick eyes was confirmed by retinoscopy and axial measurement,the accumulation and synthesis of fibronectin in sclera cartilage were determined by immunohistochemistry assay. ResultsFibronectin expression in the plasma and membrane of sclera chondrocytes as well as cartilage capsule of myopia eyes is significantly higher than that in control eyes(P<0.01).ConclusionFibronectin might regulate ocular enlargement or respond to myopiagenic mechanisms in experimental form-deprivation myopia. |
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| Keywords: | myopia fibronectin immunohistochemistry |
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