新生大鼠海马神经元原代培养方法的研究

目的 :研究和比较 3种不同培养神经元的方法 ,提供一种纯化效率和存活率均较高的培养技术。方法 :取新生SD大鼠的海马区 ,应用一般培养法、加阿糖胞苷法、加阿糖胞苷和神经生长因子法培养神经元 ,观察神经元的形态。用倒置显微镜观察和MTT比色分析检测神经元的存活率。用ABC免疫组化染色法 ,比较 3种不同培养方法在不同培养时间所获得神经元的纯度。结果 :加阿糖胞苷和神经生长因子培养组神经元生长良好 ,纯度和存活率均较高。结论 :加阿糖胞苷和神经生长因子培养神经元的方法具有简便可行 ,易于生长的优点 ,可作为神经元体外培养的良好模型

The study of primary culture methods for hippocampal neurons of newborn rats

AIM: To compare three different methods for neuron culture, so as to provide a culture technique with higher neuron purity and survival rate. METHODS: Neurons in hippocampal region of newborn SD rats were cultured by common culture method, cytosine arabinoside (Ara c) supplementing method, Ara c and nerve growth factor (NGF) supplementing method. Then morphology of neurons was observed under microscope. The survival rates of the neurons at different culture times were compared by inverted microscope observation and MTT colorimetry. The purity of neurons cultured by 3 methods was detected by immunocytochemical staining. RESULTS: Neurons cultured by Ara c and NGF supplementing method grew well. The purity and survival rate of neurons cultured by Ara c and NGF supplementing method was highest. CONCLUSION: Ara c and NGF supplementing method is a good method for neuron culture.