| 大鼠肺动脉平滑肌细胞的外向钾电流及四乙基铵对此电流作用的量效关系 |
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| 引用本文： | 张庆利,郝丽英,王丽娟,丛华,李金鸣,李智.大鼠肺动脉平滑肌细胞的外向钾电流及四乙基铵对此电流作用的量效关系[J].中国医科大学学报,1997(2). |
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| 作者姓名： | 张庆利 郝丽英 王丽娟 丛华 李金鸣 李智 |
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| 作者单位： | Department of Pharmacology，School of Basic Medical Sciences. China Medical University，Shenyang，110001 |
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| 基金项目： | 国家“八五”科技攻关项目 |
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| 摘 要： | 以全细胞膜片钳制技术观察大鼠肺动脉平滑肌细胞外向钾电流的基本特性及四乙基铵（ＴＥＡ）对其作用的量效关系。结果表明：１．将细胞膜电位钳制在－５０ｍＶ，当指令电位大于－５０ｍＶ时，可以记录到电压依赖性的外向钾电流；２．当指令电位为＋７０ｍＶ时，在１０－３ｍｏｌ／Ｌ、１０－４ｍｏｌ／Ｌ、１０－５ｍｏｌ／Ｌ和１０－６ｍｏｌ／Ｌ各浓度的四乙基铵可使外向钾电流分别降低４８．３７％、４１．２１％、２２．１５％和１０．４７％。四乙基铵对外向钾电流的作用具有浓度依赖关系
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| 关 键 词： | 大鼠 肺动脉平滑肌细胞 全细胞膜片钳制技术 外向钾电流 四乙基铵 |
Outward Potassium Current in Smooth Muscle Cell of Rat Pulmonary Artery and the Dose-response Relationship Between Tetraethylammonium and the Current |
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| Zhang Qingli,Hao Liying,Wang Lijuan,et al.Outward Potassium Current in Smooth Muscle Cell of Rat Pulmonary Artery and the Dose-response Relationship Between Tetraethylammonium and the Current[J].Journal of China Medical University,1997(2). |
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| Authors: | Zhang Qingli Hao Liying Wang Lijuan |
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| Abstract: | Outward potassium current of smooth muscle cell in rat pulmonary artery was determined and the dose-response relationship between tetraethylammonium (TEA) and the current was observed. The results showed that: 1.When cell membrane potential was held at -50 mV and command potential was larger than -50 mV, outward potassium current could be recorded. This current was voltage dependent; 2. When command potential was 70 mV, TEA of different concentration, such as 10 -3 mol/L, 10 -4 mol/L, 10 -5 mol/L, and 10 -6 mol/L, decreased this potassium current 48.37%, 41.21%, 22.15% and 10.47% respectively. The effect of TEA on outward potassium currents was concentration-related. |
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| Keywords: | rat pulmonary artery smooth muscle cell whole cell patch-clamp technique outward potassium current tetraethylammonium |
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