| 小鼠小肠上皮内淋巴细胞的提取与抗原表型测定和功能的初步研究 |
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| 引用本文： | 周正任,叶晓卉,杨晓临,王丹.小鼠小肠上皮内淋巴细胞的提取与抗原表型测定和功能的初步研究[J].中国免疫学杂志,2001,17(3):135-137. |
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| 作者姓名： | 周正任 叶晓卉 杨晓临 王丹 |
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| 作者单位： | 中国医科大学病原生物学教研室， 沈阳 110001 |
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| 基金项目： | 本文受辽宁省自然科学基金资助(962316) |
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| 摘 要： | 目的建立一种提取小肠上皮内淋巴细胞的方法，并对其功能进行初步研究。方法采用机械分离法和Percoll非连续密度梯度离心法提取小鼠小肠上皮内淋巴细胞，经间接荧光染色后用流式细胞仪检测表型。应用乳酸脱氢酶法检测其自然杀伤功能。结果应用该法可获得小鼠小肠上皮内淋巴细胞2×10
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| 关 键 词： | 小肠上皮内淋巴细胞 自然杀伤活性 抗原表型 小鼠 |
| 文章编号： | 1000-484X(2001)03-0135-03 |
Studies on isolation
phonotype and natural cytotoxicity of murine intraepithelial lymphocytes |
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| ZHOU Zheng Ren,YE Xiao Hui,YANG Xiao Lin et al.China Medical University,Pathogenic Microbiology.Studies on isolation
phonotype and natural cytotoxicity of murine intraepithelial lymphocytes[J].Chinese Journal of Immunology,2001,17(3):135-137. |
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| Authors: | ZHOU Zheng Ren YE Xiao Hui YANG Xiao Lin China Medical University Pathogenic Microbiology |
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| Institution: | ZHOU Zheng Ren,YE Xiao Hui,YANG Xiao Lin et al.China Medical University,Pathogenic Microbiology Department,Shenyang 110001 |
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| Abstract: | Objective:To extract a method for isolating murineintraepithelial lymphocy te and analyze its main function.Methods:Murine intestinal intraepithelial lymphocyte was isolated by a mechanical technique and Percoll d i scontinous density gradient centrigugation.Its surface antigens were measured by using indirect immunoflurescence.Its natural killing activity was examined by L DH method.Results:2×106～4×106iIELs/per mouse were a cquired by using this method,add its vitality＞95%.Of phenotypic antigen,more than 73. 8 % showed CD8+cell,TCRγδ 43.8%.IEL's natural killing activity was high er than PBMCs.Conclusion:This method is effective of isolating IELs.IELs has natural killing activity. |
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| Keywords: | Intraepithelial lymphocyte Natural killing activity Phonotype |
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