| 水蛭提取液对视网膜色素上皮细胞内游离钙离子的影响 |
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| 引用本文： | 郑燕林,陈海燕,吕帆,王万杰,周春阳.水蛭提取液对视网膜色素上皮细胞内游离钙离子的影响[J].中国中医眼科杂志,2007,17(1):39-41. |
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| 作者姓名： | 郑燕林 陈海燕 吕帆 王万杰 周春阳 |
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| 作者单位： | 成都中医药大学眼科,成都,610072 |
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| 摘 要： | 目的观察水蛭提取液对视网膜色素上皮细胞(retinal pigment epithelium,RPE)内游离钙离子(Intracellular liber calcium ion,Ca2 ]i)浓度的影响。方法利用体外培养的视网膜色素上皮细胞,采用双波长荧光分光光度计测量法测定视网膜色素上皮细胞内Ca2 ]i浓度。结果空白组(A)133.4517±13.17789、凝血酶组(A1)192.2037±4.54292、水蛭组(A2)96.9935±8.80148、水蛭和凝血酶同时加组(A3)142.0867±26.47195、先加水蛭后加凝血酶组(A4)162.7284±29.13950、先加凝血酶后加水蛭组(A5)130.4810±20.48673。结论水蛭提取液能使RPE细胞内游离Ca2 浓度下降,为进一步深入研究水蛭提取液抑制RPE细胞增殖的作用机制,防治增生性玻璃体视网膜病变(proliferative vitreo-retinopathy,PVR)提供了实验室依据。
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| 关 键 词： | 水蛭 视网膜色素上皮细胞 细胞内游离钙离子 |
| 文章编号： | 1002-4379（2007）01-0039-03 |
| 收稿时间： | 2006-08-28 |
| 修稿时间： | 2006-08-28 |
Effect of hirudinoid extract on intracellular liber calcium ion of retinal pigment epithelium |
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| ZHENG Yanlin,CHEN Haiyan,LU Fan,et al..Effect of hirudinoid extract on intracellular liber calcium ion of retinal pigment epithelium[J].Journal of Traditional Chinese Ophthalmology,2007,17(1):39-41. |
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| Authors: | ZHENG Yanlin CHEN Haiyan LU Fan |
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| Institution: | ZHENG Yanlin,CHEN Haiyan,LU Fan,et al.Department of Ophthalmology,the Affiliated Hospital of Chengdu Traditional Chinese Medicine University,Chengdu 610072,China |
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| Abstract: | OBJECTIVE To observe effect of percolation leech extract on intracellular liber calcium ionCa~(2 )]i of retinal pigment epithelium(RPE)cultured in vitro.METHODS Use retinal pigment epithelium(RPE)culture in vitro,to observe effect of leech extract on retinal pigment epithelium(RPE)Ca~(2 )]i.RESULTS One hundred and twenty-eight mg/ml leech extract can reduceCa~(2 )]i of retinal pigment epithelium(RPE)cultured in vitro.CONCLUSIONS Leech extract can reduceCa~(2 )]i of retinal pigment epithelium(RPE)cultured in vitro to provide experimental evidence for drug treatment on proliferative vitreoretinopathy(PVR). |
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| Keywords: | leech extract retinal pigment epithelium RPE intracellular liber calcium ion |
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