山茱萸多糖的化学研究

目的 :研究山茱萸补益活性成分。方法 :采用热水提取 ,乙醇沉淀 ,三氯醋酸除蛋白等步骤分得粗多糖 ,DEAE 纤维素及SephadexG-200柱层析分离 ,纯化 ,高效凝胶渗透色谱鉴定纯度并测定分子量 ,薄层层析 ,纸层析 ,气相色谱 ,气 质联用 ,甲基化分析 ,部分酸水解及¹³CNMR确定多糖的化学结构。结果 :分离得到一水溶性多糖Co-4 ,其分子量为 2.4 7× 10⁴,主链为 1 ,4-连接的 β-D-葡聚糖 ,部分葡萄糖残基 6位上有分枝。结论 :首次对山茱萸多糖进行较深入的化学研究并阐明水溶性多糖Co-4化学结构。

Chemical Studies on the Polysaccharide in Fructus Corni

OBJECTIVE: To study the tonic constituents in Fructus Corni, the pulp of Cornus of ficinalis. METHOD: Hot-water extraction, ethanol precipitation and column chromatography on DEAE-cellulose and Sephadex G-200 were used to isolate and purify the polysaccharides in Fructus Corni. HPGPC was used to determine the purity and measure the mean molecular weight of the polysaccharides. TLC, PC, GC, GC-MS, 13CNMR, partial acid hydrolysis and permethylation to elucidate the structures. RESULT: A water-soluble polysaccharide with mean molecular weight of 2.47 x 10(14), named Co-4, was obtained. It's main chain was (1-->4) linked beta-D-glucan, with branching points at position 6. CONCLUSION: A thorough study on the polysaccharides of Fructus Corni has been made for the first time and the structure of Co-4 has been elucidated.