三种检测方法在菌阴结核病诊断中的价值

目的 探讨实时定量聚合酶链反应（PCR）、结核抗体（TB-Ab）、结核菌素（BCG-PPD）5IU皮试三种方法联合检测对菌阴结核病患者临床诊断的意义。方法 依上述三种方法检测初治菌阴肺结核121例，结核性胸膜炎93例，肺外结核病41例，非结核疾病35例。结果 PCR、TB-Ab、PPD检测对菌阴肺结核的阳性检出率依次为41．3％、37．2％、31．4％，对结核性胸膜炎的阳性检出率依次为30．1％、43．0％、34．4％，肺外结核病组的阳性检出率依次为46．3％、39．0％、34．1％。联合检测与单项检测的最高阳性率相比，菌阴肺结核由41．3％提高到75．2％，结核性胸膜炎由43．0％提高到73．1％，肺外结核组由46．3％提高到74．3％。结论 三种方法联合检测对于菌阴结核病的诊断，具有实用意义。

Study On clinical application of 3 methods for detection of smear negative tuberculous

Objective To evaluate the diagnostic value of 3 kinds of methods combination used for smear negative tuberculous. Methods The 3 testing methods were PCR, TB-Ab and BCG-PPD 5 IU skiu test. The 3 testing methods were individually managed respectively to 121 smear negative cases, 93 tuberculous pleurisy, 41 tuberculosis out of pulmonary and 35 non- tuberculosis respiratory patients in the control group. Results The positive detection rate of single test by PCR,TB-Ab and PPD for sputum TB negative respiratory patients were 41.3%, 37.2%, 31.4%, respectively. The rates for tuberculous pleurisy patients were 30. 1%, 43.0% , 34.4% respectively. The rates for tuberculosis out of pulmonary patients were 46.3%, 39. 0%, 34. 1% respectively. Conclusion It is valuable to use the methods combined in detection of smear negative tuberculosis patients.