许旺细胞的体外培养提纯

许旺细胞是组织工程修复神经损伤的种子细胞,能否获得大量且高纯度的许旺细胞对于组织工程学至关重要.许旺细胞的培养纯化技术日趋完善,其经典的培养方法有植块法和酶消化法,在此基础上改进的去除成纤维细胞的方法有差速贴壁法、免疫选择法、阿糖胞苷补充法、刺激因子增殖法等.最近比较新的提纯方法有磁性活细胞分离法、层粘连蛋白纯化法、三维支架联合培养法.另外还有一些因子,如胎牛血清浓度等影响许旺细胞的扩增.这些技术的主要目的就是在体外获得大量的高纯度许旺细胞,以满足组织工程修复神经的需要.许旺细胞的体外培养和提纯方法众多,且已取得初步进展,但在此技术完全成熟之前尚还有许多问题有待解决,如周期长、细胞活性低、在传代培养中细胞生物学特性不稳定等.

In vitro culture and purification of Schwann cells

Schwann cells are the seed cells of tissue engineedng in the regeneration of peripheral nerve.so whether we can harvest enough Schwann cells of high purity is very important in the tissue engineering.Culture and purification technology of Schwann cells has been improved day by day.The classical cultural methods include tissue-clump cultural method and enzyme digestion cultural method.On the basis of the classical cultural methods,someone uses new technologies to delete fibroblasts,such as addition of cytosine arabinoside,addition of antimitogenic agents.The latest purified methods include magnetic activated-cells separation,co-culture with three-dimensional scaffolds and laminin-coated wells purified method.The aim of the skills above is to provide enough Schwann cells for the tissue engineering repeir of degenerated peripheral nerves.There are many ways of Schwann cells culture and purification.and we have made a primary progress,but it still needs further studies before the entire achievemenL such as long cycle,low activity in cultured calls,and the instable biological characteristics during passage culture,etc.