CA125在子宫内膜样腺癌组织中的表达特征及意义

目的: 探讨CA125（cancer antigen 125,CA125）在子宫内膜样腺癌组织中的表达特征及临床应用价值。方法: 采用免疫组织化学SP法检测50例子宫内膜样腺癌、20例子宫内膜增生过长和20例正常子宫内膜组织中的CA125的表达。根据阳性细胞百分比和阳性反应强度得出免疫反应得分（immunoreactive score， IRS）。结果: 正常内膜及子宫内膜增生过长组织中，CA125主要表达在腺腔面和基底面，子宫内膜样腺癌组织中CA125主要表达在核周的胞质中，呈粗大的颗粒。子宫内膜癌组织中的CA 125 的阳性率为82％（41/50）高于正常子宫内膜（50％）及子宫内膜增生过长（55％）, 差异有显著性（P <0.05）。CA125 IRS与子宫内膜样腺癌病理分级相关。结论:免疫组化检测CA125有助于子宫内膜良、恶性病变的区分，并对预后评估有一定意义。

Immunohistochemical characterization of CA125 in endometrial adenocarcinoma and its significance.

Objective: The aim of this study was to determine the tissue distribution of CA125 in normal, hyperplastic endometrium and endometriod adenocarcinoma, and its significance in diagnosis. Methods: CA125 were detected by the immunohistochemical staining in 50 cases of endometriod adenocarcinoma, 20 cases of endometrial hyperplasia and 20 cases of nomal endometrium. The immunohistochemical staining for CA125 was assessed according to the immunoreactive score (IRS) that evaluated the proportion of cells expressing CA125 and the intensity of staining. Results: In nomal, hyperplastic endometrium. CA125 expression was observed in luminal and basal surface of glandular epithelial cells. In endometriod adenocarcinoma, the reactivity with anti-CA 125 was in the perinunclear region of the cytoplasm as accumulations of atypical coarse granules. The positive rate of CA125 in endometriod adenocarcinoma was 82 % (41/50), and was significant higher than that in normal (50%) and hyperplastic endometrium (55.0%) (P <0.05). CA125 IRS correlated with the grade of endometriod adenocarcinoma . Conclusions: Immunohistochemical staining of CA125 may be helpful in distinguish between benign and neoplastic endometrium, and can be used to evaluate the prognosis of endometriod adenocarcinoma.