| 人结肠癌染色体1q杂合性缺失分析 |
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| 引用本文： | 黄幼生,解娜,邓晓佳,宋伟伟,罗志飞.人结肠癌染色体1q杂合性缺失分析[J].肿瘤防治研究,2011,38(6):658-662. |
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| 作者姓名： | 黄幼生 解娜 邓晓佳 宋伟伟 罗志飞 |
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| 作者单位： | 520101海口，海南医学院病理教研室 |
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| 基金项目： | 海南省教育厅基金资助项目(2007047) |
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| 摘 要： | 目的揭示人结肠癌1q杂合性缺失与临床病理特征的关系。方法应用15对微卫星标志结合PCR、聚丙烯酰胺凝胶电泳等技术检测人结肠癌染色体1q杂合性缺失。结果检测发现人结肠癌染色体1q至少1个位点存在杂合性缺失的占74.2%（69/93）；平均缺失频率为17.7％，缺失频率较高的微卫星有D1S413、D1S305等，分别为34.62%、43.75%；缺失图谱分析显示常见缺失区域位于D1S2878~D1S2346(1q21.3~1q23.2)及D1S413~D1S249(1q31.3~1q32.1)之间。最小缺失区域为D1S413~D1S249，大约7.1cM的遗传距离。1q LOH(loss of heterozygosity, LOH)频率与癌细胞分化程度相关，低分化结肠癌的LOH频率显著高于高分化及中分化结肠癌（P<0.05）。结论人结肠癌染色体1q31.3 ~1q32.1及1q21.3~1q23.2区域频繁发生LOH，在这些区域附近可能存在与结肠癌相关的抑癌基因；结肠癌1q LOH率与癌细胞分化程度相关。
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| 关 键 词： | 结肠癌 染色体1q 杂合性缺失 微卫星 |
| 收稿时间： | 2010-06-17; |
Loss of Heterozygosity on Chromosome 1q in Human Colon Carcinoma |
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| HUANG You-sheng,XIE Na,DENG Xiao-jia,SONG Wei-wei,LUO Zhi-fei.Loss of Heterozygosity on Chromosome 1q in Human Colon Carcinoma[J].Cancer Research on Prevention and Treatment,2011,38(6):658-662. |
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| Authors: | HUANG You-sheng XIE Na DENG Xiao-jia SONG Wei-wei LUO Zhi-fei |
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| Institution: | Institute of Pathology, Hainan Medical College, Haikou 570101，China |
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| Abstract: | Objective To define the minimally lost regions(MLR) on chromosome 1q,and further to explore the molecular genetics alteration during the malignant progression of human colon mucosa.MethodsFifteen microsatellite markers were used and combined with PCR to detect the frequencies of LOH of every selected microsatellite site on chromosome 1q in colon carcinoma.Results Chromosome 1q LOH was identified in 69 of 93 colon carcinoma(74.2%).The LOH values in the D1S413(34.62%) and D1S305(43.75) were higher than that i... |
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| Keywords: | Colon cancer Loss of heterozygosity Chromosome 1q Microsatellite markers |
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