饮食结构对大鼠体内氨茶碱的药动学影响

目的探讨不同饮食结构对茶碱在大鼠体内的药动学影响。方法 36只大鼠分为对照组(空腹给药)、标准饮食组(给药前给予标准饮食)和高脂饮食组(给药前给予高脂饮食),各组大鼠灌胃给予氨茶碱,采用高效液相色谱法测定各个时间点血中的茶碱浓度,并用DAS2.0软件计算药动学参数。结果对照组、标准饮食组和高脂饮食组的主要动力学参数如下:血药浓度时间-曲线面积为(305.239±68.728)、(239.927±51.837)和(245.264±53.630)μg·h-1·ml-1,平均驻留时间为(6.935±0.904)、(7.239±0.936)和(7.144±0.661)h,半衰期为(5.066±1.098)、(5.480±1.441)和(5.119±1.230)h,达峰时间为(1.167±0.389)、(1.833±0.389)和(1.917±0.289)h,清除率为(0.069±0.017)、(0.086±0.018)和(0.085±0.018)L·h·kg,峰浓度为-1-1(52.485±10.472)、(35.901±7.534)和(33.309±6.255)μg·ml-1。标准饮食和高脂饮食均可使峰浓度减少,高脂饮食可使达峰时间延长。结论饮食可使茶碱在大鼠体内的药动学参数发生改变。

Effects of different diet on pharmacokinetics of aminophylline in rats

Objective To study the effects ofdifferentdietary on pharmacokinetics ofaminophylline in rats.Methods 36 healthy rats were divided averagely into control group,standard diet group and high fat group.The pharmacokinetic parameters in rat plasma after proadministration of aminophylline were investigated by high pressure liquid chdomatography(HPLC).Results The pharmacokinetic parameters in control group,standard diet group and high fat group were as follows:AUC0-t(305.239±68.728),(239.927±51.837) and(245.264±53.630) μg·h-1·mL-1,MRT0-∞(6.935±0.904),(7.239±0.936) and(7.144±0.661) h,t1/2(5.066±1.098),(5.480±1.441) and(5.119±1.230) h,Tmax(1.167±0.389),(1.833±0.389) and(1.917±0.289)h,CL(0.069±0.017),(0.086±0.018) and(0.085±0.018) L·h-1·kg-1,Cmax(52.485±10.472),(35.901±7.534) and(33.309±6.255) μg·mL-1.Cmax was lower in both standard diet and high fat groups than in control group,and Tmax was delayed in high fat group.Conclusion Both of the standard diet and high fat had the effect on pharmacokinetics of aminophylline in plasma.