种植尿道黏膜细胞的可降解尿道支架重建战伤后尿道的形态学研究

目的:比较种植尿道黏膜上皮细胞的网状生物可降解尿道支架与单纯螺旋形尿道支架重建战伤后尿道狭窄的组织相容性及形态学变化。方法:成年新西兰雄兔18只,取原代培养兔尿道上皮细胞,种植于网状生物可降解尿道支架上,进行形态学鉴定及染色,观察存活状态。将种植尿道黏膜细胞的支架与单纯尿道支架,植入战伤性尿道狭窄动物模型,观察两种尿道支架重建损伤后尿道的组织相容性及形态学改变。结果:培养的尿道黏膜上皮细胞在网丝孔隙中黏附、增殖状态良好。结论:种植尿道黏膜上皮细胞的网状生物可降解尿道内支架,具有良好的修复重建战创伤后尿道狭窄的功能,有望成为修复重建战伤性尿道狭窄的有效方法。

Morphological study of war wound urethral stricture reconstructed with biodegradable urethral stent with autologons urethral epithelial cells

Objective: To compare the biocompatibility properties and histological analysis of a new mesh biodegradable stent with autologous urethral epithelial cells to the spiral biodegradable stent in a war wound(or traumatic) rabbit urethral strictures model.Methods: The animal urethral epithelial cells were cultured by biopsy in vitro and seeded to the outer layer of the mesh biodegradable stent.The 18 male New Zealand white rabbits were anaesthetized and the two stents inserted into the urethra.The rabbits were killed at different sample time to analyse the effects.Results: The cultured cells adhere to fibers and growing very well.Conclusion: The mesh biodegradable stent with autologous urethral epithelial cells seem to have more advantages and function well in a war wound(or traumatic) rabbit urethral strictures model.