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| 反相离子对色谱法测定不同规格益母草中盐酸益母草碱的含量 | |
| 引用本文： | 陈碧莲,祝明,黄卫国,郭增喜. 反相离子对色谱法测定不同规格益母草中盐酸益母草碱的含量[J]. 中国药品标准, 2011, 12(2): 111-113 |
| 作者姓名： | 陈碧莲 祝明 黄卫国 郭增喜 |
| 作者单位： | 1. 浙江省食品药品检验所,杭州,310014 2. 浙江中医药大学,杭州,310053 |
| 基金项目： | 国家十一五"重大新药创立"专项 |
| 摘 要： | 目的:建立了反相离子对色谱法测定益母草中盐酸益母草碱含量的方法。方法:Agilent SB-C18柱(4.6 mm×250 mm,5μm);流动相:乙腈-0.4%辛烷磺酸钠的0.1%磷酸溶液(24∶76);检测波长:277 nm;流速:1.0 mL.min-1。结果:盐酸益母草碱进样量在0.028 66~1.433μg范围内呈良好的线性关系,平均回收率为98.0%,RSD为0.6%。结论:此方法简便、灵敏度高,重现性好,可用于益母草的质量控制。 |
| 关 键 词： | 反相离子对色谱 益母草 盐酸益母草碱 |
Determination of Leonurine Hydrochloride in Different Specifications of Herba Leonuri by Reversed-Phase Ion-Pair Chromatography |
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| Chen Bi-lian,Zhu Ming,Huang Wei-guo and Guo Zeng-xi. Determination of Leonurine Hydrochloride in Different Specifications of Herba Leonuri by Reversed-Phase Ion-Pair Chromatography[J]. , 2011, 12(2): 111-113 | |
| Authors: | Chen Bi-lian Zhu Ming Huang Wei-guo Guo Zeng-xi |
| Affiliation: | Chen Bi-lian1,Zhu Ming1,Huang Wei-guo2,Guo Zeng-xi1(1.Zhejiang Institute for Food and Drug Control,Hangzhou 310004,2.Zhejiang University of Traditional Chinese Medicine.Hangzhou 310053) |
| Abstract: | Objective： A method was developed for determination of leonurine hydrochloride in Herba Leonuri by reversed-phase ion-pair chromatography. Methods： The separation was performed on an Agilent SB-C18 column （4.6 mm ×250 mm,5μm） with acetonitrile-0. 4% sodium octanesulfonate of 0. 1% phosphoric acid solution （24： 76） as mobile phase at a flow rate of 1.0 mL·min-1 The detection wavelength was 277 nm. Results： There was a good linear relationship with the range of 0. 028 66-1. 433μg. The average recovery was 98.0% （ RSD =0. 6% ） . Conclusion： The method is simple, sensitive and reliable, it can be used as quantitative analysis method for Herba Leonuri. |
| Keywords: | Reversed-phase ion-pair chromatography Leonurine hydrochloride Herba Leonuri |
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