毛囊混合细胞在胶原/壳聚糖多孔支架上重建毛囊样结构

目的探讨利用毛囊混合细胞植入胶原／壳聚糖多孔支架内体外重建毛囊的可行性。方法用滴加法或注射法将体外培养的C57BL／6J近交系乳鼠背部皮肤毛囊混合细胞以不同传代数、不同细胞密度接种至胶原／壳聚糖多孔支架，倒置显微镜下观察支架表面或浅层的细胞生长或毛囊形成情况。将支架经10％甲醛固定后行组织学观察（H-E染色）。另用共聚焦激光扫描显微镜观察支架内活细胞的生长以及毛囊样结构的形成。结果在一定传代次数和细胞密度下，在支架内可形成具有毛干的毛囊样结构。激光共聚焦扫描显微镜发现团块内细胞排列呈同心圆状，整个三维结构似一长颈花瓶，且该结构仅见于注射法接种细胞的支架内。结论毛囊混合细胞植入胶原／壳聚糖多孔支架内体外可形成具有毛干的毛囊样结构。

Reconstruction of hair follicle-like structure with hair follicle cells on collagen/chitosan porous scaffolds

Objective To investigate the feasibility of in vitro reconstruction of hair follicles by implanting hair follicle cells onto collagen/chitosan porous scaffolds. Methods The cultured hair follicle cells isolated from the dorsal skin of 4-day old C57BL/6 mice were implanted in different density and at different passages into collagen/chitosan porous scaffolds or onto the scaffold surface in vitro, using dropping or injection method. The proliferation and assembly of the follicular cells on the surface of collagen/chitosan porous scaffold were observed by an inverted microscope. The histologic characteristic of the scaffolds was observed with hematoxylin and eosin stain after fixation with 10% formaldehyde. The growth of cells and formation of hair follicles were assessed by confocal laser scaning microscopy. Results Hair follicle-like structure with hair stem could form in collagen/chitosan porous scaffolds implanted with hair follicle cells in a certain density and at a certain times of passage. Condusion Hair follicle-like structure can generate from collagen/chitosan porous scaffolds implanted with hair follicle cells in vitro.