| 高效液相色谱法测定蝎子七中没食子酸的含量 |
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| 引用本文： | 王汉平,杨志灵,蒋军.高效液相色谱法测定蝎子七中没食子酸的含量[J].中国药房,2005,16(10):780-781. |
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| 作者姓名： | 王汉平 杨志灵 蒋军 |
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| 作者单位： | 1. 陕西省汉中市中医院,汉中市,723000
2. 陕西汉王药业有限公司,汉中市,723000 |
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| 摘 要： | 目的:建立以高效液相色谱法测定蝎子七中没食子酸含量的方法。方法:色谱柱为Nova -PakC18,流动相为0.2 %甲醇乙腈溶液-0.1 %磷酸-0.15 %三乙胺(3∶50∶47) ,流速为1.0ml/min ,检测波长为271nm。结果:没食子酸进样量在0.028μg～0.14μg范围内线性关系良好(r=0. 9991) ,平均加样回收率为99.05 % (RSD=0.40 % )。结论:本方法简便、快速、准确、重现性好,可用于蝎子七中没食子酸的含量测定。
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| 关 键 词： | 高效液相色谱法 蝎子七 没食子酸 含量测定 |
| 文章编号： | 1001-0408(2005)10-0780-02 |
| 修稿时间： | 2004年8月23日 |
Determination of Gallic Acid in Viviparous Bistort Rhizome by HPLC |
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| Wang Hanping,Yang Zhiling,JIANG Jun.Determination of Gallic Acid in Viviparous Bistort Rhizome by HPLC[J].China Pharmacy,2005,16(10):780-781. |
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| Authors: | Wang Hanping Yang Zhiling JIANG Jun |
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| Abstract: | OBJECTIVE:To determine the content of gallic acid in viviparous bistort rhizome by HPLC.METHODS:The chromatographic column was Nova-Pak C 18 and the mobile phase was0.2%methanol methyl cyanide solution-0.1%phosˉphoric acid-0.15%triethylamin(3∶50∶47),the flow rate was1.0ml/min and the detection wavelength was271nm.REˉSULTS:It took a good linear relationship when the sample size of gallic acid was within the range of0.028～0.14μg(r=0.9991),the average recovery was99.05%(RSD=4.0%).CONCLUSION:The method is convenient,fast,reproducible,which can be used for the content determination of gallic acid in viviparous bistort rhizome. |
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| Keywords: | HPLC Viviparous bistort rhizome Gallic acid Content determination |
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