C反应蛋白基因多态性与缺血性脑卒中的关系

目的探讨超敏C反应蛋白(hs-CRP)水平及rs1130864基因多态性与脑梗死(CI)的相关性。方法采用病例对照研究,对105例脑梗死患者和121例健康对照者进行研究。采用免疫透射比浊法测定hs-CRP,用多聚酶链式反应(PCR)和限制性片段长度多态性测定rs1130864基因多态性。患者入院后立即用美国国立卫生院神经功能缺损评分(National Institutes of Health Stroke Scale,NIHSS)对其进行神经功能测评。结果脑梗死组与对照组rs1130864基因型频率分布统计学无显著性差异(P>0.05)。rs1130864与脑梗死病情无关联。脑梗死多因素回归方程的主要危险因素包括高血压史、糖尿病史、吸烟史、HDL-C,hs-CRP水平。结论 rs1130864(1444C>T)基因多态性与脑梗死无关联。

Association between C-reactive protein gene polymorphism and cerebral infarction

Objective To explore the relationship between the C-reactive protein levels,rs1130864 gene polymorphism and cerebral infarction.Methods A case-control study on 105 patients with cerebral infarction and 121 healthy controls was carried out.The high-sensitive C reactive protein was determination by immune-turbidimetry,the genotype and allele frequencies of rs1130864 gene polymorphism were assayed by polymerase chain reaction-restricted fragments length polymorphism(PCR-RFLP).All the patients were measured with National Institutes of Health Stroke Scale(NIHSS).Results There were no statistical significant for rs1130864 genotype frequencies between the patients with cerebral infarction and the healthy controls(P > 0.05),and there were no statistical significant difference that neither CRP genotypes nor alleles showed an association with patient’s condition(P > 0.05).The risk factors for cerebral infarction,including history of hypertension,diabetes,smoking,HDL-C,hs-CRP level.Conclusion rs1130864 polymorphism is not associated with cerebral infarction in Chines.