| HPLC法测定鹿蹄草中槲皮素的含量 |
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| 引用本文： | 张文,黎钰铃,封丽彬,范卫锋. HPLC法测定鹿蹄草中槲皮素的含量[J]. 中国中医药信息杂志, 2009, 16(9): 47-48 |
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| 作者姓名： | 张文 黎钰铃 封丽彬 范卫锋 |
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| 作者单位： | 广西玉林制药有限责任公司,广西,玉林,537001 |
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| 摘 要： | 目的建立HPLC法测定鹿蹄草中槲皮素含量的方法。方法采用HPLC法，色谱柱：hgilent Eclipse XDB—C18柱（4．6mm×150mm，5μm）；流动相：甲醇-0．1％磷酸（50：50）；检测波长：370nm。结果槲皮素在0．092～0．46Pg范围内线性关系良好，平均回收率为99．24％，RSD=0．70％。结论方法简便、准确、灵敏度高、重复性好，能有效测定鹿蹄草药材中槲皮素含量。
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| 关 键 词： | 鹿蹄草 槲皮素 高效液相色谱法 含量测定 |
Content Determination of Quercetin in Pyrola cailiatha H.Andres by HPLC |
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| Affiliation: | ZHANG Wen, LI Yu-ling, FENG Li-bin, et al (Guangxi Yulin Pharmaceutical Co.Ltd., Yulin 537001, China) |
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| Abstract: | Objective To set up a method for determining the quercetin in Pyrola calliatha H. Andres. Method HPLC method was carried on Agilent Eclipse XDB-C18 column （4.6 mm× 150 mm, 5 μm） with mobile phase of methanol-0.1% phosphoric acid （50 ： 50）, and the detecting wavelength was at 370 nm. Result The standard curve was linear within the range of 0.092-0.46 μg. The average recovery rate was 99.24% and RSD was 0.70%. Conclusion The method is simple, accurate, highly sensitive and reproducible. It can be used for the quality determination of quercetin in Pyrola calliatha H. Andres. |
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| Keywords: | Pyrola calliatha H.Andres quercetin HPLC content determination |
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