BcL—2和C—fos在卵巢上皮性肿瘤中的表达及意义

探讨抗凋亡基因ＢｃＬ２ 和原癌基因Ｃｆｏｓ 在卵巢上皮性肿瘤发生发展中的作用及其临床意义，采用单克隆抗体免疫组化技术对４２ 例卵巢上皮组织进行ＢｃＬ２ 和Ｃｆｏｓ 蛋白表达的检测。８ 例正常卵巢组织均未检测出二基因产物的表达。ＢｃＬ２ 和Ｃｆｏｓ 在良、恶性卵巢肿瘤组织中检出率分别为２０％ （２／１０）、５９－４％ ；３０％ （３／１０）、４６－９％ 。ＢｃＬ２ 阳性在良性恶性组织间差异显著（Ｐ＜０－０５）。二基因产物表达均随病理分级升高而增加，高中分化与低分化比较差异均极显著（Ｐ＜０－０１）。ＢｃＬ２ 阳性提示较好的预后（Ｐ＜０－０１）。Ｃｆｏｓ 阳性预后欠佳（Ｐ＜０－０５）。二基因产物表达无相关性。结果表明，ＢｃＬ２ 和Ｃｆｏｓ 在卵巢上皮性肿瘤的发生、发展、分化中起作用。ＢｃＬ２ 过表达预后较好，Ｃｆｏｓ 过表达预后差，二基因产物表达可作为临床预后评估指标。

Expression of BcL 2 and C fos onco proteins in ovarian epithelial tumor and their clinicopathological significance

To study apoptosis antagonizing protein BcL 2 and proto oncogene protein C fos expression and distribution in ovarian epithelial tumor,expression of BcL 2 and C fos protein was examined by immunohistochemistry in 42 epithelial tumors of ovary.No positive staining of BcL 2 and C fos was observed in 8 normal tissues.BcL 2 positive rate in benign and malignant tissues was 20.0%(2/10) and 59.4% respectively ( P <0.05).C fos protein expression rate was 30.0%(3/10) and 46.9% respectively ( P >0.05).BcL 2 and C fos protein expression was correlated with low histologic grade ( P <0.01).BcL 2 positive carcinomas had a statistically significantly better outcome ( P <0.01) and C fos positive suggested poor prognosis ( P <0.05).No relationship was found between two gene expression.The results suggest that BcL 2 and C fos may play a role in the pathogenesis and development of ovarian epithelial tumor.Expression of BcL 2 protein was associated with improved survial.C fos protein expression was correlated with poor prognosis.