Behaviour of <Emphasis Type="Italic">Sinapis alba</Emphasis> chromosomes in a <Emphasis Type="Italic">Brassica napus</Emphasis> background revealed by genomic <Emphasis Type="Italic">in-situ</Emphasis> hybridization |
| |
| Authors: | Y?P?Wang X?X?Zhao K?Sonntag P?Wehling Email author" target="_blank">R?J?SnowdonEmail author |
| |
| Institution: | 1.College of Bioscience and Biotechnology,Yangzhou University,Yangzhou,China;2.Federal Centre for Breeding Research on Cultivated Plants,Institute of Agricultural Crops,Gro? Lüsewitz,Germany;3.Institute for Plant Breeding and Crop Science I,Justus Liebig University,Giessen,Germany |
| |
| Abstract: | Genomic in-situ hybridization (GISH) was applied to study the behaviour of addition chromosomes in first and second backcross (BC) progenies
of hybrids between Brassica napus ssp. napus L. (AACC, 2n = 38) and Sinapis alba L. (SS, 2n = 24) produced by electrofusion. With GISH using genomic DNA of S. alba was used as probe it was possible to clearly distinguish both of the parental genomes and effectively monitor the fate of
S. alba chromosomes in the BC1 and BC2 progenies. GISH analysis confirmed the sesquidiploid genome composition (AACCS) of the BC1 progenies, which contained 38 chromosomes from B. napus and 12 chromosomes from S. alba. Genome painting in the pollen mother cells (PMCs) of the BC1 plants revealed intergenomic association between B. napus and S. alba chromosomes, whereby a maximum of 4 trivalents between AC and S chromosomes were identified at metaphase I. In the BC2 progenies, aneuploids with different numbers of additional chromosomes from S. alba, ranging from 1 to 7, were confirmed. Three putative monosomic alien addition lines were characterized, and the results are
discussed with respect to the potential for intergenomic chromosome recombination. |
| |
| Keywords: | Brassica napus L genomic in-situ hybridization (GISH) hybrid progeny Sinapis alba L |
| 本文献已被 SpringerLink 等数据库收录！ |
|
