匹鲁卡品癫痫模型慢性期大鼠海马苔藓纤维出芽的观察

目的观察腹腔注射匹鲁卡品后大鼠痫性发作及海马苔藓出芽。方法 Wistra大鼠60只随机分为模型组和对照组,每组30只,均进行Nissl染色及Timm银染。并于实验当天行脑电图检查。结果腹腔注射匹鲁卡品后大鼠可见急性癫痫发作,观察慢性期自发性发作。Nissl染色示模型组各区评分两两比较,结果示CA3、门区与CA1区神经元缺失差异有统计学意义（P〈0.05）。Timm银染示2组苔藓纤维出芽Timm银染颗粒评分比较,差异有统计学意义（P〈0.05）。结论 腹腔注射匹鲁卡品简单复制了人类癫痫的基本病理特征,是研究癫痫的一种简便方法。

The observation of mossy fiber sprouting in hippocampus zone of rat epilepsy models induced by pilocarpine

Objective To investigate the onset of rats epilepsy after pilocarpine injection and mossy fiber sprouting in hippocampus zone.Methods 60 rats were randomly divided into two groups:epilepsy group and control group,30 rats in each group.The neuronal damage,mossy fiber sprouting were detected by Nissl and neo-Timm staining.The ECG was performed in the day of experiment for all the rats.Results After the rats were injected with pilocarpine,93% of them developed epilepsy,and most of them showed spontaneous onset during the chronic period.Timm stainning results showed that there was a significant difference in the neuron deletion in epilepsy group between CA3 region and dentate hilus region,and between CA3 region and CA1 region(P<0.05),and there was a significant difference in silver staining granules scoring of mossy fiber sprouting between epilepsy group and control group(P<0.05).Conclusion The epilepsy models can be induced by pilocarpine injection in rats,with basic pathological characteristics of human epilepsy,which is a useful and simple way for studying epilepsy.