急性髓系白血病细胞端粒酶活性及端粒长度的同步分析

目的探讨急性髓系白血病(AML)患者端粒酶活性、端粒长度的变化及意义.方法采用TRAP-ELISA-PAGE法测定端粒酶表达活性,Southernblot方法测定端粒长度.结果AML患者端粒酶活性[吸光度(A)值为2.298±1.059]较正常对照(A值为0.387±0.598)显著增高,同时其平均端粒长度[(7.6±2.1)kb]较正常对照[(9.3±1.9)kb]明显缩短.端粒长度的变化主要发生在端粒酶活性高表达的白血病细胞中.结论AML细胞端粒酶活化可能与其端粒缩短有关.端粒的丢失可导致染色体的失稳,激活端粒酶依赖的端粒机制,在白血病的发生中起着重要作用.

Analysis of telomerase activity and telomere length in acute myelogenous leukemia

OBJECTIVE: To study the changes and significance of telomerase activity and telomere length in acute myelogenous leukemia. METHODS: TRAP-ELISA-PAGE was used to detect telomerase activity, Southern blot to estimate the length of telomere. RESULTS: Telomerase activity was significantly higher in AML(Absorption(A): 2.298 +/- 1.059) than in normal control(A: 0.387 +/- 0.598) and the mean telomere length of AML [(7.6 +/- 2.1) kb] was significantly shorter than that of normal control[(9.3 +/- 1.9) kb]. The alteration of the telomere length was detected mainly in telomerase-positive AML. CONCLUSIONS: It suggested that there was a close relationship between telomerase activity and telomere length in AML. The activation of telomerase might play an important role in the genesis and development of AML and telomere length changes may correlate with the activation of telomerase.