促红细胞生成素与促红细胞生成素受体在大鼠脱离视网膜中的表达

目的 观察促红细胞生成素（EPO）和促红细胞生成素受体（EPOR）在大鼠脱离视网膜中的表达情况。方法48只雄性SD大鼠，随机分为正常对照组、视网膜脱离（RD）后1、3、6、12、24、48、72 h组，每组6只大鼠12只眼。视网膜下腔单次注射1.4%透明质酸钠致上半侧视网膜隆起建立RD模型，采用逆转录聚合酶链式反应（RT-PCR）和蛋白免疫印迹（Western blot）测定不同时间点EPO和EPOR的mRNA和蛋白水平的表达情况，同时采用免疫组织化学方法观察EPO和EPOR在视网膜中定位表达的情况。结果 EPO和EPOR的mRNA表达水平在RD后均上调，均于RD后48 h达到高峰，分别于RD后6、12 h显著高于正常对照组，差异具有统计学意义（P＜0.05）；同样，EPO和EPOR的蛋白表达水平也在RD后均增高并在RD后48 h达到高峰，均于RD后3 h显著高于正常对照组，差异具有统计学意义（P＜0.05）。免疫组织化学结果显示正常视网膜自神经节细胞层至光感受器细胞的内外节均有EPO弱表达，RD后48 h视网膜相应部位呈强阳性表达；正常视网膜自神经节细胞层至光感受器细胞的内节均有EPOR表达，RD后48 h视网膜相应部位呈强阳性着染。结论 RD后大鼠视网膜EPO和EPOR表达均逐渐增强，48 h达到高峰；大鼠神经视网膜大部分层次能表达EPO和EPOR。

Expression of erythropoietin and erythropoietin receptor in detached retina in rat model

Objective To observe the expression of erythropoietin (EPO) and EPO receptor (EPOR) in detached retina in rat model.Methods Fourty-eight male SD rats were randomly divided into control group and retinal detachment (RD) groups (1 hour,3,6,12,24,48,and 72 hours group) with 6 rats (12 eyes) in each group.1.4% hyaluronic acid was slowly injected into the subretinal space to induce the detachment of the upper retina to set up the RD model.The expression levels of mRNA and protein of EPO and EPOR were measured by RT-PCR and western-blotting analysis.Meanwhile,the locations of EPO and EPOR in retina were checked by immunohistochemistry.Results Both of the mRNA and protein levels of EPO and EPOR increased after RD,and reached the peak at the 48th hour after RD.The mRNA levels of EPO and EPOR were significantly higher in the 6 and 12 hours group than that in the control group(P< 0.05).The protein levels of EPO and EPOR were significantly higher in 3 hours group than that in the control group(P<0.05).Immunohistochemistry indicated weak expression of EPO from ganglion cell layer to inner and outer segment of photoreceptor cells,and strong expression in the corresponding location was found 48 hours after RD.Expression of EPOR from ganglion cell layer to inner segment of photoreceptor cells in the normal retina was detected,and strong expression in the corresponding location was found 48 hours after RD.Conclusion The expression of EPO and EPOR in retina increases gradually after RD,and reaches the peak at the 48th hour;most of the layers of neural retina can express EPO and EPOR.