豚鼠耳蜗微血管内皮细胞的分离培养与鉴定

目的：建立稳定可靠的耳蜗微血管内皮细胞分离培养方法。方法：采用 显微解剖法分离出耳蜗血管纹，组织块培养法进行体外培养。结果：按种后2d，部分组织块边缘有散在的细胞生长，之后细胞数逐渐增多，10d左右以组织块为中心可见成片细胞组成的细胞集落。倒置显微镜下，单个培养细胞多呈长梭形，而融合成片状单层的培养细胞排列紧密，有内皮细胞培养时典型的“铺路石样”外观。经纯化后，95％以上的培养细胞第VIII因子相关抗原显示阳性反应。结论：本研究方法能够获取体外培养的耳蜗微血管内皮细胞。

Isolation, culture and characterization of cochlea microvascular endothelial cells from guinea pigs

Objective　To set up a reliable method for isolating and culturing cochlea microvascular endothelial cells. Methods　Cochlea stria was separated by micrergy from guinea pigs and the stria tissue nubbles were cultured in vitro. Results　Two days later, a few cells were seen disseminately around partial tissue nubbles and their number was increased with time prolongation. There were some cellular colonies consisted of large quantity of cells around the partial tissue nubbles on 10 th day. Under the inversion microscope, the single cultured cells presented a long fusiform and the confluent monolayer cells linked tightly and exhibited the typical structure of “scree” of cultured endothelial cells in vitro. After purification, over 95% of the cultured cells showed factorⅧ relative antigen positive reaction. Conclusion　The method used in this study can obtain cochlea microvascalar endothelial cells of guinea pig in vitro.