三颗针植物中生物碱分布状态的研究

目的:建立梯度洗脱双波长检测的HPLC法,同时测定小檗碱、药根碱、小檗胺、巴马汀含量的方法,并应用该法考察小檗属植物中生物碱的分布状态.方法:采集甘肃不同产地小檗属植物的茎木、根木、茎皮、根皮样品,用本文建立的HPLC法分析.结果:小檗碱、药根碱、小檗胺、巴马汀线性范围分别为:0.028μg～4.74μg(r=0.9998);0.012μg～2.0μg(r=0.9996);0.026μg～0.52μg(r=0.9999);0.015μg～2.56μg(r=0.9998).结论:不同产地、不同部位三颗针植物中生物碱的含量分布有明显的差异.

Distribution of four alkaloids in plants of Berberis

In order to assess a simple and effective method for sensitive detection of alkaloids in Berberis plants, HPLC with gradiation elution is described. This method was developed for determination of four kinds of alkaloids including berbamine, jatrorrhizine, berberine and palmatine in methanolic extracts from bark and core of root and stem of Berberis. The linear correlations of berberine, jatrorrhizine, berbamine and palmatine were in the range of 0.028 - 4.74 microg (r = 0.9998), 0.012 - 2.0 microg (r = 0.9996), 0.026 - 0.52 microg (r = 0.9999), 0.015 - 2.56 microg (r = 0.9998), respectively. The content of alkaloids had obvious difference in Berberis plants from different habitats and different parts.