帕金森大鼠黑质外液和红细胞内Mg2+含量的变化

目的:观察帕金森病(PD)大鼠中脑黑质外液及红细胞内Mg~(2+)浓度的改变。方法:采用6-羟基多巴(6-OHDA)损毁制备偏侧PD大鼠模型24只,分4组各6只,分别于造模后第7、14、21、28天采集大鼠动脉血中的红细胞,采用微透析技术收集中脑黑质细胞外液,火焰原子吸收光谱法测定红细胞内和黑质细胞外液Mg~(2+)含量。选5只健康大鼠作为对照组,进行相同测定。结果:各时点PD组红细胞内Mg~(2+)含量均显著低于对照组(P<0.05),且随病程延长而逐渐下降(P<0.05);但PD组中脑黑质细胞外液Mg~(2+)含量仅在第21、28天明显低于对照组(P<0.05)。结论:Mg~(2+)缺乏与PD的发生及病程进展有一定关联。

Changes in Magnesium Levels in RBC and Extracelluar Fluid of Substantia Nigra in a Rat Model of Parkinson''s Disease

Objective:To explored changes in magnesium levels in red blood cells and extracelluar fluid of sub- stantia nigra in a rat model of Parkinson's disease.Methods:Twenty-four rats were stereotactically injected with 6- OHDA to establish the unilateral model of Parkinson's disease.Blood samples and extracellular fluid of the substan- tia nigra were extracted from six PD rats on the 7th,14th,21st and 28th days after the injection.Contents of magne- sium were detected with atom-absorption spectrophotometer technique.Samples of 5 normal rats were subjected to the same assay as controls.Results:When compared to the control group,the magnesium concentrations in RBC decreased significantly in a time dependent manner (P<0.05),whereas those in the extracellular fluid of the sub- stantia nigra have decreased statistically only on the 21st,28th days in PD rats (P<0.05).Conclusion:Deficiency of magnesium correlates with the pathogenesis of Parkinson's diseases to some extent.