Immunoprotective activities of a Streptococcus suis pilus subunit in murine models of infection |
| |
| Authors: | Manuela Garibaldi,Manuel J. Rodrí guez-Ortega,Francesca Mandanici,Angela Cardaci,Angelina Midiri,Salvatore Papasergi,Orazio Gambadoro,Vittorio Cavallari,Giuseppe Teti,Concetta Beninati |
| |
| Affiliation: | 1. The Elie Metchnikoff Department, University of Messina, Messina I-98125, Italy;2. Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, Córdoba 14071 Spain;3. Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba 14071, Spain;4. Dipartimento di Patologia Umana, University of Messina, Messina I-98125, Italy |
| |
| Abstract: | ![]()
Pili of gram-positive bacteria are key virulence factors and their subunits are considered excellent vaccine candidates. Streptococcus suis is an emerging zoonotic agent that can cause epidemics of life-threatening infections in humans, but the functional role or immunoprotective potential of its pilus components have not been studied yet. Using a selective proteomics approach, we have identified a surface protein of serotype 2 S. suis showing features of an ancillary pilus subunit, as evidenced by bioinformatics analysis, immunoblot and immunoelectron microscopy. Immunization with recombinant fragments of this protein, designated herein as PAPI-2b, markedly protected mice from systemic S. suis infection. |
| |
| Keywords: | Streptococcus suis Pili Proteomics |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
