Direct estimation of the frequency of human cytotoxic T lymphocytes and their precursors following in vitro allosensitization |
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Authors: | D J Bryan C E Pinto T C Fuller |
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Institution: | Blood Transfusion Service and the Department of Surgery, Harvard Medical School at the Massachusetts General Hospital, Boston, USA |
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Abstract: | Cell mediated lympholysis (CML) has been proposed as an in vitro model of the rejection process that results from transplantation of allogeneic tissue. To date, the absolute frequencies of cytotoxic T lymphocytes (CTL) and their precursors (CTL.P) have not been directly estimated in man because of technical difficulties. Through optimizing the conditions for radiometric detection of 51Cr release and the attendant improvement in CML sensitivity, direct CTL frequency estimates have been determined in peripheral blood (PBL), spleen (SPL), and lymph nodes (LNC) after in vitro allostimulation using unrelated human cells and limiting dilution assays. The mean frequency of CTL generated from PBL is 1 in 826 cells (0.121% +/- 0.101%) which, from preliminary experiments, is significantly greater than that generated from either LNC or SPL (p less than 0.05). With restimulation of primed cells on day 10, the frequency of CTL generated from PBL was increased 400%. The CTL.P frequency (0.0064% +/- 0.0050%) was approximately 5% of the corresponding CTL frequency. The CTL.P frequencies were found to be minimal estimates as both accessory "filler" cells and T cell growth factors increased the level of detection of CTL.P an average of threefold. The limiting cell dilution assay as detailed in this report should be a powerful tool for defining the cellular requirements and related factors necessary for optimal induction of a CTL response and should provide the means for determination of the immunogenetic requirements and the allospecificity of human cytotoxic lymphocytes. |
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Keywords: | CTL cytotoxic T lymphocytes PHA phytohemagglutinin CTL P precursors of CTL SPL splenic lymphocytes CML cell-mediated lympholysis TC chromium-51-labeled phytohemagglutinin lymphoblast target cells LNC lymph node cells TCGF T-cell growth factor (Interleukin II) MHC major histocompatibility complex MLC mixed lymphocyte culture PBL peripheral blood lymphocytes |
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