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| RhoA和蛋白激酶A对胃癌细胞迁移的影响 | |
| 引用本文： | 王瑛,陈永昌. RhoA和蛋白激酶A对胃癌细胞迁移的影响[J]. 江苏大学学报(医学版), 2005, 15(1): 1-4 |
| 作者姓名： | 王瑛 陈永昌 |
| 作者单位： | 江苏大学医学院生理学教研室,江苏,镇江,212001;江苏大学医学院生理学教研室,江苏,镇江,212001 |
| 基金项目： | 国家自然科学基金资助项目(30340035 30470891)江苏大学高级人才启动基金资助项目 |
| 摘 要： | 目的：研究RhoA对胃癌细胞迁移的作用和蛋白激酶A对这一作用的影响。方法：采用Boyden槽迁移率分析法和刮除一迁移分析法，观察RhoA激动剂LPA及PKA激动剂cAMP对人胃癌细胞SGC-7901迂移活动的影响。结果：加入RhoA激动剂LPA后，癌细胞迁移活动明显增强。加入PKA激动剂cAMP后再加入RhoA激动剂LPA，癌细胞迁移活动不再出现明显变化。结论：RhoA活化可促进SGC-7901细胞迁移，PKA可通过抑制RhoA活性而阻止该细胞迁移。 |
| 关 键 词： | RhoA 蛋白激酶A 胃癌细胞 迁移 |
| 文章编号： | 1671-7783(2005)01-0001-04 |
| 修稿时间： | 2004-09-29 |
The Influence of PKA and RhoA on Migration of Gastric Cancer Cells |
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| WANG Ying,CHEN Yong-chang. The Influence of PKA and RhoA on Migration of Gastric Cancer Cells[J]. Journal of Jiangsu University Medicine Edition, 2005, 15(1): 1-4 | |
| Authors: | WANG Ying CHEN Yong-chang |
| Abstract: | Objective: This experiment was designed to study the action of RhoA on gastric cancer cell migration and the influence of PKA on this action. Methods: Boyden chamber motility assay and scrape-motility assay were applied and the effects of RhoA stimulatant LPA and PKA stimulatant cAMP on migration of human gastric cancer cell SGC -7901 were investigated. Results: The migration of cells stimulated with RhoA stimulatant LPA was obviously increased. In cells stimulated with cAMP and then LPA, the migration did not change significantly. Conclution: RhoA could promote cancer cell migration, whereas PKA could hold back cancer cell migration through suppressing RhoA activation. |
| Keywords: | RhoA PKA Gastric cancer cells Migration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |